蛋氨酸亚砜亚胺和蛋氨酸砜对产气克雷伯菌中谷氨酸合成的影响。
Effect of methionine sulfoximine and methionine sulfone on glutamate synthesis in Klebsiella aerogenes.
作者信息
Brenchley J E
出版信息
J Bacteriol. 1973 May;114(2):666-73. doi: 10.1128/jb.114.2.666-673.1973.
Abstract
At least two pathways exist in Klebsiella aerogenes for glutamate synthesis. A mutant blocked in one pathway due to the loss of glutamate dehydrogenase (gltD) does not require glutamate and has the same growth characteristics as the parent strain in most media; however, its growth is inhibited by the analogues methionine sulfoximine and methionine sulfone. Wild-type Klebsiella is resistant to 0.1 M methionine sulfoximine or methionine sulfone, whereas the gltD mutant is sensitive to 1 mM concentrations. Either glutamate or glutamine is effective in overcoming this inhibition. Activities of both glutamine synthetase and glutamate synthetase, two enzymes involved in the second pathway of glutamate synthesis, are inhibited by methionine sulfoximine and methionine sulfone. The primary effect of methionine sulfoximine appears to be the prevention of glutamine production necessary for subsequent glutamate synthesis via glutamate synthetase enzyme.
摘要
产气克雷伯菌中至少存在两条谷氨酸合成途径。由于谷氨酸脱氢酶（gltD）缺失而在一条途径中受阻的突变体不需要谷氨酸，并且在大多数培养基中具有与亲本菌株相同的生长特性；然而，其生长受到类似物甲硫氨酸亚砜胺和甲砜霉素的抑制。野生型产气克雷伯菌对0.1M甲硫氨酸亚砜胺或甲砜霉素具有抗性，而gltD突变体对1mM浓度敏感。谷氨酸或谷氨酰胺均可有效克服这种抑制作用。参与谷氨酸合成第二条途径的两种酶——谷氨酰胺合成酶和谷氨酸合成酶的活性均受到甲硫氨酸亚砜胺和甲砜霉素的抑制。甲硫氨酸亚砜胺的主要作用似乎是阻止通过谷氨酸合成酶随后合成谷氨酸所需的谷氨酰胺的产生。
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