P22转导中的一些改进方法。
Some improved methods in P22 transduction.
作者信息
Hartman P E
出版信息
Genetics. 1974 Apr;76(4):625-31. doi: 10.1093/genetics/76.4.625.
Abstract
Recent refinements simplify methods for P22 transduction in Salmonella and allow improved recovery of phage-free transductional clones. The methods include use of: integration- and lysis-defective phage mutants, heat-killed bacteria to eliminate free phage, direct plating of phage and bacteria, replica-plating for detection of phage content of individual clones, improved broth for phage growth, and procurement of high titer phage from P22 lysogens.
摘要
最近的改进简化了沙门氏菌中P22转导的方法，并提高了无噬菌体转导克隆的回收率。这些方法包括使用：整合和裂解缺陷型噬菌体突变体、热灭活细菌以消除游离噬菌体、噬菌体和细菌的直接平板接种、用于检测单个克隆噬菌体含量的影印平板接种、用于噬菌体生长的改良肉汤，以及从P22溶原菌中获取高滴度噬菌体。
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