Cancer Cell Death laboratory, part of LabEX DEVweCAN, Cancer Initiation and Tumoral Cell Identity Department, CRCL, U1052 INSERM, UMR CNRS 5286, Centre Léon Bérard, Université Lyon I, Institut Convergence PLAsCAN Lyon, France; Institut Universitaire de France (IUF), Paris, France.
Cancer Cell Death laboratory, part of LabEX DEVweCAN, Cancer Initiation and Tumoral Cell Identity Department, CRCL, U1052 INSERM, UMR CNRS 5286, Centre Léon Bérard, Université Lyon I, Institut Convergence PLAsCAN Lyon, France.
Semin Cell Dev Biol. 2024 Mar 15;156:58-65. doi: 10.1016/j.semcdb.2023.07.003. Epub 2023 Jul 10.
Membrane structural integrity is essential for optimal mitochondrial function. These organelles produce the energy needed for all vital processes, provided their outer and inner membranes are intact. This prevents the release of mitochondrial apoptogenic factors into the cytosol and ensures intact mitochondrial membrane potential (ΔΨ) to sustain ATP production. Cell death by apoptosis is generally triggered by outer mitochondrial membrane permeabilization (MOMP), tightly coupled with loss of ΔΨ . As these two processes are essential for both mitochondrial function and cell death, researchers have devised various techniques to assess them. Here, we discuss current methods and biosensors available for detecting MOMP and measuring ΔΨ , focusing on their advantages and limitations and discuss what new imaging tools are needed to improve our knowledge of mitochondrial function.
膜结构完整性对于优化线粒体功能至关重要。这些细胞器产生所有生命过程所需的能量，前提是它们的内外膜完整。这可以防止线粒体凋亡原性因子释放到细胞质中，并确保完整的线粒体膜电位（ΔΨ）以维持 ATP 产生。细胞凋亡通常由外线粒体膜通透性（MOMP）触发，与 ΔΨ 的丧失紧密相关。由于这两个过程对于线粒体功能和细胞死亡都是必不可少的，因此研究人员设计了各种技术来评估它们。在这里，我们讨论了用于检测 MOMP 和测量 ΔΨ 的当前方法和生物传感器，重点讨论了它们的优点和局限性，并讨论了需要哪些新的成像工具来提高我们对线粒体功能的认识。
