Li He, Zhang Letian, Li Xingtai, He Haocheng, Fu Guoan, Zhu Yi Zhun, Hu Wei, Qiu Lige, Gong Liang, Zhang Youming
School of Pharmacy, Faculty of Medicine and Faculty of Chinese Medicine, State Key Laboratory of Quality Research in Chinese Medicines, Laboratory of Drug Discovery from Natural Resources and Industrialization, Macau University of Science and Technology, Macau, China.
Shenzhen Key Laboratory of Genome Manipulation and Biosynthesis, Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China.
Front Pharmacol. 2025 May 30;16:1545356. doi: 10.3389/fphar.2025.1545356. eCollection 2025.
(CP) is one of the most popular Qi-invigorating herbal medicines and has been extensively used to promote health and vitality in China for a long time. polysaccharide (CPP) is the principal active components of CP, which is considered as the reason for CP widespread application. However, it has not been revealed that CPP exert a Qi-invigoration effect by protecting mitochondria and/or improving mitochondrial function in the existing traditional Chinese medicine theories.
We extracted CPP from and investigated the effects of CPP on energy metabolism and mitochondrial protection.
Based on the mice chronic hypoxia model for imitating the energy deficiency state of the human body, which was administered with CPP by oral gavage daily for 10 days, mitochondrial permeability transition (MPT), lipid peroxidation product malondialdehyde (MDA) in brain, mitochondrial respiratory function, the levels of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) in liver cells were assayed. Adenylate energy charge (AEC), total adenylate pool (TAP), ATP/ADP, and ATP/AMP ratios were calculated.
CPP can inhibit the formation of MDA in mice brains, decrease oxygen consuming rate and respiratory control ratio (RCR) of liver mitochondria, increase levels of ATP, TAP and AEC in liver cells under chronic hypoxia condition.
CPP can possess and improve mitochondrial energy metabolism and bioenergetic levels.
党参是最常用的补气草药之一，长期以来在中国被广泛用于促进健康和活力。党参多糖（CPP）是党参的主要活性成分，被认为是党参广泛应用的原因。然而，在现有的中医理论中，尚未揭示CPP通过保护线粒体和/或改善线粒体功能发挥补气作用。
我们从党参中提取了CPP，并研究了CPP对能量代谢和线粒体保护的作用。
基于模拟人体能量缺乏状态的小鼠慢性缺氧模型，每天经口灌胃给予CPP，持续10天，检测线粒体通透性转换（MPT）、脑内脂质过氧化产物丙二醛（MDA）、线粒体呼吸功能、肝细胞中三磷酸腺苷（ATP）、二磷酸腺苷（ADP）和一磷酸腺苷（AMP）的水平。计算腺苷酸能荷（AEC）、总腺苷酸池（TAP）、ATP/ADP和ATP/AMP比值。
CPP可抑制小鼠脑内MDA的形成，降低肝线粒体的氧消耗率和呼吸控制率（RCR），在慢性缺氧条件下增加肝细胞中ATP、TAP和AEC的水平。
CPP可维持并改善线粒体能量代谢和生物能量水平。
