Prinz M, Boll K, Baum H, Shaler B
Department of Forensic Biology, Office of the Chief Medical Examiner, New York, NY 10022, USA.
Forensic Sci Int. 1997 Mar 14;85(3):209-18. doi: 10.1016/s0379-0738(96)02096-8.
A combination of four Y-specific polymorphic STR loci was amplified simultaneously using fluorescently labeled primers. Multiplex conditions required optimization to eliminate constant bands and amplification products for female DNA. A series of experiments was carried out for mixtures of DNA from two males, and from male and female individuals for the Y-specific STRs and an autosomal locus. For the male/male mixtures amplified with the Y specific system, and amplified for an autosomal locus, the minor component in the mixture could only be identified up to a ratio of 1:10, 1:50 respectively. In male/female DNA mixtures the Y STR alleles could be identified for the highest ratio tested, 400 pg male in DNA in 800 ng female DNA which amounts to a ratio of 1:2000.
使用荧光标记引物同时扩增四个Y特异性多态性STR位点的组合。需要优化多重条件以消除女性DNA的恒定条带和扩增产物。对来自两名男性以及男性和女性个体的DNA混合物进行了一系列实验，以检测Y特异性STR和一个常染色体位点。对于用Y特异性系统扩增并针对常染色体位点进行扩增的男性/男性混合物，混合物中的次要成分分别只能在1:10、1:50的比例下被识别。在男性/女性DNA混合物中，对于测试的最高比例，即在800 ng女性DNA中含有400 pg男性DNA（比例为1:2000），可以识别出Y STR等位基因。
