Faculty of Medicine and Health, School of Pharmacy, The University of Sydney, Sydney, Australia.
Pharmaceutical Biology, Department Pharmaceutical Sciences, University of Basel, Basel, Switzerland.
J Pharm Pharmacol. 2023 Oct 5;75(10):1357-1365. doi: 10.1093/jpp/rgad066.
Annona muricata, also known as graviola, is traditionally used for the treatment of a range of disorders including cancer. Interest in A. muricata use has increased in recent years. This study investigated the quality and safety of a selection of commercially available A. muricata leaf products.
Seven commercially available products were purchased via online shopping sites. Each product was assessed for quality indicators including weight variation, quantification of the bioactive constituent annonacin, presence of annonaceous acetogenins and contaminants. The samples were evaluated by thin-layer chromatography, high-performance liquid chromatography, liquid chromatography-mass spectroscopy, low-resolution mass spectrometry and nuclear magnetic resonance spectroscopy. Microbial analysis was carried out in accordance with the British Pharmacopoeia. Heavy metals were analysed by inductive coupled plasma mass spectrometry.
Of the seven products analysed, one product contained less than half of the content stated on the label. The labelled dosage recommendation varied between products. There was a high variation in annonacin concentration (1.05-3.09 mg/g) and the presence of annonaceous acetogenins. One of the products was found to have a total aerobic microbial count above the United States Pharmacopoeia limit.
The variation in the indicators of quality and safety of commercially available A. muricata leaf products tested have implications for clinicians and people living with cancer who use these herbal products.
俗称“神秘果”的南美番荔枝，传统上被用于治疗多种疾病，包括癌症。近年来，人们对南美番荔枝的应用兴趣日益增加。本研究调查了一系列市售的南美番荔枝叶产品的质量和安全性。
通过网上购物网站购买了 7 种市售产品。对每个产品的质量指标进行评估，包括重量变化、生物活性成分安诺宁的定量分析、是否存在番荔枝乙酰酯酶和污染物。采用薄层色谱法、高效液相色谱法、液相色谱-质谱法、低分辨率质谱法和核磁共振波谱法进行样品评估。微生物分析按照英国药典进行。采用电感耦合等离子体质谱法分析重金属。
在所分析的 7 种产品中，有一种产品的含量不到标签上标明的一半。标签上的推荐剂量在产品之间存在差异。安诺宁浓度（1.05-3.09mg/g）和番荔枝乙酰酯酶的存在存在很大差异。其中一种产品的总需氧微生物计数超过美国药典的限量。
所测试的市售南美番荔枝叶产品的质量和安全性指标的变化，对使用这些草药产品的临床医生和癌症患者都有影响。
