Hunan Institute of Advanced Sensing and Information Technology, Xiangtan University, Xiangtan, 411105, China.
Hunan Institute of Advanced Sensing and Information Technology, Xiangtan University, Xiangtan, 411105, China; Foshan Green Intelligent Manufacturing Research Institute of Xiangtan University, Guangdong, 528311, China.
Biosens Bioelectron. 2023 Oct 1;237:115525. doi: 10.1016/j.bios.2023.115525. Epub 2023 Jul 8.
Laser-scribed graphene (LSG), a promising electrode material has attracted special research interest in recent years. Here, the fabricating process-electrochemical property correlation of laser-scribed graphene (LSG) devices was discussed emphatically and a pertinent optimization was performed to achieve better electroanalytical performance. Experiment results indicated that the laser scribing technique possessed great process latitude and reducing laser power and scribing speed facilitated fabricating high-quality graphene electrodes. Benefiting from its binder-free 3D porous network structure and high active/geometric area ratio, the optimized LSG electrode was superior to the screen-printed carbon electrode (SPCE) on electrochemical performance in the [Fe(CN)] redox system. Integrating the LSG electrode with a homemade hand-held detector, a portable electrochemical sensing platform with smartphone readout was developed. It realized a specific detection of HO (linear range: 0.02-3.4 mM, sensitivity: 24.56 μA mM cm), glucose (linear range: 0.04-4.0 mM, sensitivity: 16.35 μA mM cm) by directly decorating biological enzymes without artificial redox mediator and featured a satisfactory comprehensive performance. The constructed immunosensor for tumor necrosis factor-α exhibited a wide linear range (2-500 pg mL) and a 4.3-fold enhancement in sensitivity compared with that of SPCE. With satisfactory selectivity, reproducibility, and sensitivity, the developed smartphone-based electrochemical sensing platform held great promise in accurate detection on the spot. This work also provided a significant reference for tailoring binder-free carbonaceous electrode materials toward the desired application.
激光刻蚀石墨烯（LSG）作为一种很有前途的电极材料，近年来引起了人们的特别关注。本文重点讨论了激光刻蚀石墨烯（LSG）器件的制造工艺-电化学性能相关性，并进行了相关优化，以获得更好的电分析性能。实验结果表明，激光刻蚀技术具有很大的工艺灵活性，降低激光功率和刻蚀速度有利于制备高质量的石墨烯电极。得益于其无粘结剂的 3D 多孔网络结构和高活性/几何面积比，优化后的 LSG 电极在[Fe(CN)]氧化还原体系中的电化学性能优于丝网印刷碳电极（SPCE）。将 LSG 电极与自制的手持探测器集成在一起，开发了一种具有智能手机读取功能的便携式电化学传感平台。它通过直接修饰生物酶而无需人工氧化还原介体，实现了对 HO（线性范围：0.02-3.4 mM，灵敏度：24.56 μA mM cm）和葡萄糖（线性范围：0.04-4.0 mM，灵敏度：16.35 μA mM cm）的特异性检测，具有令人满意的综合性能。构建的肿瘤坏死因子-α免疫传感器具有较宽的线性范围（2-500 pg mL）和比 SPCE 高 4.3 倍的灵敏度。该基于智能手机的电化学传感平台具有良好的选择性、重现性和灵敏度，有望实现现场的精确检测。这项工作为定制无粘结剂碳质电极材料以满足特定应用提供了重要参考。
