Laboratory of Process Engineering and Environment, Faculty of Sciences and Techniques, Hassan II University of Casablanca, P.A. 149, Mohammedia 28810, Morocco.
Department of Analytical Chemistry, Institute of Research on Electron Microscopy and Materials (IMEYMAT), Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Polígono del Río San Pedro S/N, 11510 Puerto Real, Cádiz, Spain.
Biosensors (Basel). 2023 Jun 25;13(7):678. doi: 10.3390/bios13070678.
The authors present a novel sensing platform for a disposable electrochemical, non-enzymatic glucose sensor strip at physiological pH. The sensing material is based on dendritic gold nanostructures (AuNs) resembling feather branches, which are electrodeposited onto a laser-scribed 3D graphene electrode (LSGE). The LSGEs were fabricated via a one-step laser scribing process on a commercially available polyimide sheet. This study investigates several parameters that influence the morphology of the deposited Au nanostructures and the catalytic activity toward glucose electro-oxidation. The electrocatalytic activity of the AuNs-LSGE was evaluated using cyclic voltammetry (CV), linear sweep voltammetry (LSV), and amperometry and was compared to commercially available carbon electrodes prepared under the same electrodeposition conditions. The sensor demonstrated good stability and high selectivity of the amperometric response in the presence of interfering agents, such as ascorbic acid, when a Nafion membrane was applied over the electrode surface. The proposed sensing strategy offers a wide linear detection range, from 0.5 to 20 mM, which covers normal and elevated levels of glucose in the blood, with a detection limit of 0.21 mM. The AuNs-LSGE platform exhibits great potential for use as a disposable glucose sensor strip for point-of-care applications, including self-monitoring and food management. Its non-enzymatic features reduce dependence on enzymes, making it suitable for practical and cost-effective biosensing solutions.
作者提出了一种新颖的基于树枝状金纳米结构（AuNs）的电化学非酶葡萄糖传感器条，可在生理 pH 值下使用。传感材料基于类似于羽毛分支的树枝状 AuNs，其通过激光刻蚀 3D 石墨烯电极（LSGE）上的一步激光刻蚀工艺沉积。LSGE 是通过在市售聚酰亚胺片上的一步激光刻蚀工艺制造的。本研究研究了影响沉积 Au 纳米结构形态和葡萄糖电氧化催化活性的几个参数。通过循环伏安法（CV）、线性扫描伏安法（LSV）和安培法评估了 AuNs-LSGE 的电催化活性，并与在相同电沉积条件下制备的市售碳电极进行了比较。当在电极表面施加 Nafion 膜时，传感器在存在干扰剂（如抗坏血酸）的情况下表现出良好的安培响应稳定性和高选择性。所提出的传感策略提供了广泛的线性检测范围，从 0.5 到 20 mM，涵盖了血液中的正常和升高的葡萄糖水平，检测限为 0.21 mM。AuNs-LSGE 平台具有作为用于即时护理应用（包括自我监测和食品管理）的一次性葡萄糖传感器条的巨大潜力。其非酶特性降低了对酶的依赖，使其成为实用且具有成本效益的生物传感解决方案的理想选择。
