Synthesis of hollow molecular imprinting nanoparticles based on polyethylenimine and boronate affinity for selective extraction of ovalbumin.

The hollow MCM-48 polyethyleneimine carboxyphenylboronic acid molecularly imprinted polymers (H-MPC@MIPs) were synthesized to efficiently and selectively separate and enrich the ovalbumin (OVA) in egg white samples. Polyethyleneimine contained enough active amino groups to increase the amount of boric acid molecules modified to silica nanoparticles. Meanwhile, the materials were etched to enhance the adsorption effect. The H-MPC@MIPs exhibited a rapid adsorption equilibrium rate (within 30 min) and outstanding adsorption capacity for OVA (1334.1 mg g). It possessed a good reusability after 5 cycles. In addition, both the high density and the imprinting action of boric acid were essential for enhancing the identification and binding of OVA. The OVA in egg white samples was successfully selectively enriched using this method.