Li Shu-Fang, Wu Jia-Hui, Hu Teng-Gen, Wu Hong
School of Food Science and Engineering, South China University of Technology, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou, China.
Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China.
Int J Biol Macromol. 2023 Sep 1;248:125797. doi: 10.1016/j.ijbiomac.2023.125797. Epub 2023 Jul 11.
In order to efficiently improve the colon-targeted delivery of quercetin, the hydrophobic core-shell nanofibers were fabricated to encapsulate quercetin using ethyl cellulose as the shell and zein as the core by coaxial electrospinning. The encapsulation efficiency of coaxial nanofibers reached >97 %. FTIR and XRD results revealed the interactions between quercetin and wall materials and quercetin was encapsulated in an amorphous state. The thermal stability and surface hydrophobicity of coaxial nanofibers were improved compared to the uniaxial zein fibers. After in vitro gastrointestinal digestion, the quercetin release from core-shell nanofibers was <12.38 %, while the corresponding value for zein fibers was 36.24 %. DPPH and FRAP assays showed that there was no significant difference in the antioxidant activity of quercetin before and after encapsulation. Furthermore, the encapsulated quercetin exhibited similar anti-proliferative activity against HCT-116 cells compared to the free form. The results suggest these coaxial nanofibers have potential applications in functional foods.
为了有效提高槲皮素的结肠靶向递送能力，采用同轴静电纺丝法制备了以乙基纤维素为壳、玉米醇溶蛋白为核的疏水核壳纳米纤维来包封槲皮素。同轴纳米纤维的包封效率达到>97%。傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）结果揭示了槲皮素与壁材之间的相互作用，且槲皮素以无定形状态被包封。与单轴玉米醇溶蛋白纤维相比，同轴纳米纤维的热稳定性和表面疏水性得到了提高。体外胃肠消化后，核壳纳米纤维中槲皮素的释放率<12.38%，而玉米醇溶蛋白纤维的相应值为36.24%。二苯基苦味酰基自由基（DPPH）和铁离子还原抗氧化能力（FRAP）测定表明，包封前后槲皮素的抗氧化活性没有显著差异。此外，与游离形式相比，包封的槲皮素对人结肠癌细胞系HCT-116细胞表现出相似的抗增殖活性。结果表明，这些同轴纳米纤维在功能性食品中具有潜在的应用价值。
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