Pereira Geovana M, Manuli Erika R, Coulon Laurie, Côrtes Marina F, Ramundo Mariana S, Dromenq Loïc, Larue-Triolet Audrey, Raymond Frédérique, Tourneur Carole, Lázari Carolina Dos Santos, Brasil Patricia, Filippis Ana Maria Bispo de, Paranhos-Baccalà Glaucia, Banz Alice, Sabino Ester C
Instituto de Medicina Tropical, Faculdade de Medicina da Universidade de São Paulo, São Paulo 05403-000, Brazil.
Faculdade de Medicina da Universidade Municipal de São Caetano do Sul, São Paulo 09521-160, Brazil.
Diagnostics (Basel). 2023 Jul 7;13(13):2306. doi: 10.3390/diagnostics13132306.
Chikungunya (CHIK) is a debilitating mosquito-borne disease with an epidemiology and early clinical symptoms similar to those of other arboviruses-triggered diseases such as dengue or Zika. Accurate and rapid diagnosis of CHIK virus (CHIKV) infection is therefore challenging. This international study evaluated the performance of the automated VIDAS anti-CHIKV IgM and IgG assays compared to that of manual competitor IgM and IgG ELISA for the detection of anti-CHIKV IgM and IgG antibodies in 660 patients with suspected CHIKV infection. Positive and negative agreements of the VIDAS CHIKV assays with ELISA ranged from 97.5% to 100.0%. The sensitivity of the VIDAS CHIKV assays evaluated in patients with a proven CHIKV infection confirmed reported kinetics of anti-CHIKV IgM and IgG response, with a positive detection of 88.2-100.0% for IgM ≥ 5 days post symptom onset and of 100.0% for IgG ≥ 11 days post symptom onset. Our study also demonstrated the superiority of ELISA and VIDAS assays over rapid diagnostic IgM/IgG tests. The analytical performance of VIDAS anti-CHIKV IgM and IgG assays was excellent, with a high precision (coefficients of variation ≤ 7.4%) and high specificity (cross-reactivity rate ≤ 2.9%). This study demonstrates the suitability of the automated VIDAS anti-CHIKV IgM and IgG assays to diagnose CHIKV infections and supports its applicability for epidemiological surveillance and differential diagnosis in regions endemic for CHIKV.
基孔肯雅热（CHIK）是一种由蚊子传播的使人衰弱的疾病，其流行病学特征和早期临床症状与登革热或寨卡等其他虫媒病毒引发的疾病相似。因此，准确快速地诊断基孔肯雅病毒（CHIKV）感染具有挑战性。这项国际研究评估了自动化的VIDAS抗CHIKV IgM和IgG检测方法与手动竞争IgM和IgG酶联免疫吸附测定（ELISA）方法在检测660例疑似CHIKV感染患者的抗CHIKV IgM和IgG抗体方面的性能。VIDAS CHIKV检测方法与ELISA的阳性和阴性一致性范围为97.5%至100.0%。在经证实感染CHIKV的患者中评估的VIDAS CHIKV检测方法的灵敏度证实了报告的抗CHIKV IgM和IgG反应动力学，症状出现后≥5天IgM的阳性检测率为88.2 - 100.0%，症状出现后≥11天IgG的阳性检测率为100.0%。我们的研究还证明了ELISA和VIDAS检测方法优于快速诊断IgM/IgG检测。VIDAS抗CHIKV IgM和IgG检测方法的分析性能出色，具有高精度（变异系数≤7.4%）和高特异性（交叉反应率≤2.9%）。这项研究证明了自动化的VIDAS抗CHIKV IgM和IgG检测方法适用于诊断CHIKV感染，并支持其在CHIKV流行地区用于流行病学监测和鉴别诊断。
