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Rev Inst Med Trop Sao Paulo. 2019 Aug 19;61:e40. doi: 10.1590/S1678-9946201961040.
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Molecular epidemiology of dengue, yellow fever, Zika and Chikungunya arboviruses: An update.登革热、黄热病、寨卡病毒病和基孔肯雅热虫媒病毒的分子流行病学：最新进展
Acta Trop. 2019 Feb;190:99-111. doi: 10.1016/j.actatropica.2018.11.010. Epub 2018 Nov 13.
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Harmonization of nucleic acid testing for Zika virus: development of the 1 World Health Organization International Standard.寨卡病毒核酸检测的标准化：世界卫生组织国际标准品1的研制
Transfusion. 2017 Mar;57(3pt2):748-761. doi: 10.1111/trf.14026. Epub 2017 Feb 23.
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AIDS Rev. 2016 Jan-Mar;18(1):54-5.
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Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection.用于泛登革热和基孔肯雅病毒检测的单反应实时逆转录聚合酶链反应的临床评估
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Zika virus outbreak in Brazil.巴西的寨卡病毒疫情。
J Infect Dev Ctries. 2016 Feb 28;10(2):116-20. doi: 10.3855/jidc.8217.
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The Zika outbreak of the 21st century.21世纪的寨卡疫情。
J Autoimmun. 2016 Apr;68:1-13. doi: 10.1016/j.jaut.2016.02.006. Epub 2016 Feb 28.
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Prolonged detection of Zika virus RNA in urine samples during the ongoing Zika virus epidemic in Brazil.在巴西持续的寨卡病毒疫情期间，尿液样本中寨卡病毒RNA的长期检测。
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巴西公共实验室网络用于检测和鉴别血液中寨卡病毒、登革热病毒和基孔肯雅病毒的分子检测方法的分析性能和临床性能

Analytical and clinical performance of molecular assay used by the Brazilian public laboratory network to detect and discriminate Zika, Dengue and Chikungunya viruses in blood.

作者信息

Ribeiro Marisa O, Godoy Daniela T, Fontana-Maurell Marcela, Costa Elaine M, Andrade Elisabete F, Rocha Daniele R, Ferreira Antônio G P, Brindeiro Rodrigo, Tanuri Amilcar, Alvarez Patrícia

机构信息

Oswaldo Cruz Foundation/Fiocruz, Institute of Technology in Immunobiology Bio-Manguinhos, Rio de Janeiro, RJ, Brazil; Federal University of Rio de Janeiro, Institute of Biology, Rio de Janeiro, RJ, Brazil.

Oswaldo Cruz Foundation/Fiocruz, Institute of Technology in Immunobiology Bio-Manguinhos, Rio de Janeiro, RJ, Brazil.

出版信息

Braz J Infect Dis. 2021 Mar-Apr;25(2):101542. doi: 10.1016/j.bjid.2021.101542. Epub 2021 Feb 13.

DOI:10.1016/j.bjid.2021.101542
PMID:33587923
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9392074/
Abstract

In response to the Zika epidemics in Brazil, the ZDC molecular assay (Bio-Manguinhos) was developed and registered at the Brazilian Regulatory Agency of Health Surveillance - ANVISA. The circulation of Zika (ZIKV) Dengue (DENV) and Chikungunya (CHIKV) viruses and their clinical similarities are challenges to correctly diagnose these viruses. The simultaneous detection of ZIKV, DENV and CHIKV is an important tool for diagnosis and surveillance. Here, we present the analytical and clinical performance evaluation of ZDC molecular assay (Bio-Manguinhos) at the public health laboratories three years after its registration at ANVISA. The clinical performance demonstrates the ZDC molecular assay (Bio-Manguinhos) has 100% sensitivity and 100% specificity to detect and discriminate ZIKV, CHIKV, and DENV from clinical plasma samples. The ZDC molecular assay (Bio-Manguinhos) results were highly reproducible and no cross-reactivity was seen during testing with a panel of other infectious agents. In conclusion, the ZDC molecular assay (Bio-Manguinhos) is an accurate and reliable tool to monitor Zika, dengue and chikungunya infections in countries like Brazil with simultaneous circulation of the three viruses.

摘要

为应对巴西的寨卡疫情，巴西生物制品开发中心（Bio-Manguinhos）研发了ZDC分子检测法，并在巴西卫生监督管理局（ANVISA）进行了注册。寨卡病毒（ZIKV）、登革热病毒（DENV）和基孔肯雅病毒（CHIKV）的传播及其临床症状的相似性给这些病毒的准确诊断带来了挑战。同时检测ZIKV、DENV和CHIKV是诊断和监测的一项重要工具。在此，我们展示了ZDC分子检测法（Bio-Manguinhos）在巴西卫生监督管理局注册三年后在公共卫生实验室中的分析性能和临床性能评估。临床性能表明，ZDC分子检测法（Bio-Manguinhos）在从临床血浆样本中检测和鉴别ZIKV、CHIKV和DENV方面具有100%的灵敏度和100%的特异性。ZDC分子检测法（Bio-Manguinhos）的结果具有高度可重复性，并且在与一组其他感染因子进行测试时未观察到交叉反应。总之，对于像巴西这样三种病毒同时传播的国家，ZDC分子检测法（Bio-Manguinhos）是监测寨卡、登革热和基孔肯雅感染的准确且可靠的工具。