Zhou Luli, Lin Yuhuan, Chang Ye, Abouelezz Khaled Fouad Mohammed, Zhou Hanlin, Wang Jian, Hou Guanyu, Wang Dingfa
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Animal Health Supervision Institute of Hainan Province, Haikou 571100, China.
Animals (Basel). 2023 Jun 25;13(13):2108. doi: 10.3390/ani13132108.
In the poultry industry, there is an urgent need to evaluate and introduce natural, effective, and safe alternatives for synthetic antibiotics, which have been banned in most countries. The present study aimed to investigate the effects of dietary supplementation with extract (PSE) on the growth performance, intestinal barrier function, and metabolism of growing chickens. A total of 400 seven-day-old female chicks were randomly assigned to four dietary treatments, each of which consisted of five replicates and twenty birds each. The four experimental treatments were fed a basal diet containing 0, 100, 200, and 300 mg PSE/kg (BC, PSE1, PSE2, and PSE3 groups), respectively. The experiment lasted for 28 days. The results showed that dietary supplementation with PSE had no significant effects on the final body weight, average daily gain (ADG), average daily feed intake (ADFI), and the ratio of ADFI to ADG (F/G) ( > 0.05). Compared with the BC group, dietary supplementation with 200-300 mg/kg PSE increased the villus height in the jejunum and ileum of chickens ( < 0.05). The PSE-treated groups significantly increased the mRNA expression of , , and in the ileal mucosa of chickens ( < 0.05). In addition, a significant decrease in ileal TNF-α and IL-8 mRNA expression ( < 0.05) and a significant increase in IL-22 ( < 0.05) were observed in the PSE2 treatment compared to the BC group. Additionally, three gut metabolites (i.e., citrate, isocitrate, and spermine) showed significant differences among treatments ( < 0.05) and were involved in the tricarboxylic acid (TCA) cycle, the transfer of acetyl groups into mitochondria, and spermidine and spermine biosynthesis, respectively. In conclusion, the findings obtained here indicate that supplemental PSE can enhance the anti-inflammatory capacity and intestinal mucosal barrier function of chickens.
在家禽行业，迫切需要评估并引入天然、有效且安全的合成抗生素替代品，因为合成抗生素在大多数国家已被禁用。本研究旨在探讨日粮中添加提取物（PSE）对生长鸡生长性能、肠道屏障功能和代谢的影响。总共400只7日龄雌性雏鸡被随机分配到四种日粮处理组，每组包含五个重复，每个重复20只鸡。四种实验处理分别饲喂含0、100、200和300 mg PSE/kg的基础日粮（BC、PSE1、PSE2和PSE3组）。实验持续28天。结果表明，日粮中添加PSE对最终体重、平均日增重（ADG）、平均日采食量（ADFI）以及ADFI与ADG之比（F/G）均无显著影响（P>0.05）。与BC组相比，日粮中添加200 - 300 mg/kg PSE可增加鸡空肠和回肠的绒毛高度（P<0.05）。PSE处理组显著增加了鸡回肠黏膜中、和的mRNA表达（P<0.05）。此外，与BC组相比，PSE2处理组回肠TNF-α和IL-8 mRNA表达显著降低（P<0.05），IL-22显著增加（P<0.05）。另外，三种肠道代谢物（即柠檬酸、异柠檬酸和精胺）在处理组间显示出显著差异（P<0.05），它们分别参与三羧酸（TCA）循环、乙酰基向线粒体的转移以及亚精胺和精胺的生物合成。总之，本研究结果表明，补充PSE可增强鸡的抗炎能力和肠道黏膜屏障功能。
