Department of Infectious Diseases, Tongde Hospital of Zhejiang Province, No. 234 Gucui Road, Xihu District, Hangzhou, 321012, China.
BMC Complement Med Ther. 2020 Jun 5;20(1):175. doi: 10.1186/s12906-020-02957-w.
To explore the protective effect and mechanisms of Ixeris denticulate water extract (IDWE) in the development of liver cirrhosis in experimental rat.
Sixty rats were randomly divided into five groups: control group, model group and IDWE (2, 4 and 8 g/kg) treatment groups. Alanine transferase (ALT), aspartate transaminase (AST), albumin (ALB), tumor necrosis factor-alpha (TNF-α), Interleukin (IL)-6 and IL-8 in serum and superoxide dismutase (SOD), malondialdehyde (MDA) in liver tissue were evaluated, respectively. The liver index, liver morphology and liver histopathological analysis were detected as a supportive data. The liver protein expression of Bcl-2 and Bax were assessed by western blot, and NF-κB p65 protein expression was determined by immunohistochemistry analysis.
The result showed that a significantly decrease in the levels of serum AST, ALT and serum inflammatory factors TNF-α, IL-6 and IL-8 in IDWE-treated rats. The levels of serum ALB and SOD in liver tissue were markedly increased after IDWE treated, compared with model rats. Furthermore, IDWE-treated group also exhibited a down-regulated protein expression of NF-κB p65 and Bax, up-regulated Bcl-2 protein expression.
IDWE could effectively alleviate the course of liver cirrhosis in rat model, which may be a potent hepatoprotective agent in clinical therapy in the future.
探讨菊三七水提物（IDWE）在实验性大鼠肝硬化发展中的保护作用及其机制。
60 只大鼠随机分为 5 组：对照组、模型组和 IDWE（2、4 和 8g/kg）治疗组。分别检测血清丙氨酸转氨酶（ALT）、天门冬氨酸转氨酶（AST）、白蛋白（ALB）、肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-6 和 IL-8 以及肝组织中超氧化物歧化酶（SOD）、丙二醛（MDA）。检测肝指数、肝形态和肝组织病理分析作为辅助数据。采用 Western blot 法检测肝组织中 Bcl-2 和 Bax 的蛋白表达，免疫组化法检测 NF-κB p65 蛋白表达。
结果表明，IDWE 治疗组大鼠血清 AST、ALT 及血清炎症因子 TNF-α、IL-6 和 IL-8 水平显著降低。与模型组相比，IDWE 治疗组大鼠血清 ALB 和肝组织 SOD 水平明显升高。此外，IDWE 治疗组还表现出 NF-κB p65 和 Bax 蛋白表达下调，Bcl-2 蛋白表达上调。
IDWE 能有效缓解大鼠肝硬化的病程，有望成为未来临床治疗的有效肝保护剂。
