Gao Yuan, Song Weiguo, Hao Fei, Duo Lei, Zhe Xiaoshu, Gao Chunyan, Guo Xudong, Liu Dongjun
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.
Animals (Basel). 2023 Jul 7;13(13):2234. doi: 10.3390/ani13132234.
Cashmere, a keratinised product of secondary hair follicles (SHFs) in cashmere goats, holds an important place in international high-end textiles. However, research on the complex molecular and signal regulation during the development and growth of hair follicles (HFs), which is essential for the development of the cashmere industry, is limited. Moreover, increasing evidence indicates that non-coding RNAs (ncRNAs) participate in HF development. Herein, we systematically investigated a competing endogenous RNA (ceRNA) regulatory network mediated by circular RNAs (circRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) in skin samples of cashmere goat embryos, using whole-transcriptome sequencing technology. We obtained 6468, 394, and 239 significantly differentially expressed mRNAs, circRNAs, and miRNAs, respectively. These identified RNAs were further used to construct a ceRNA regulatory network, mediated by circRNAs, for cashmere goats at a late stage of HF development. Among the molecular species identified, miR-184 and fibroblast growth factor (FGF) 10 exhibited competitive targeted interactions. In secondary HF dermal papilla cells (SHF-DPCs), miR-184 promotes proliferation, inhibits apoptosis, and alters the cell cycle via the competitive release of FGF10. This study reports that FGF10 and its interaction with ncRNAs significantly affect SHF-DPCs, providing a reference for research on the biology of HFs in cashmere goats and other mammals.
羊绒是绒山羊次级毛囊（SHFs）的角质化产物，在国际高端纺织品中占有重要地位。然而，对于毛囊（HFs）发育和生长过程中复杂的分子和信号调控的研究有限，而这对羊绒产业的发展至关重要。此外，越来越多的证据表明非编码RNA（ncRNAs）参与毛囊发育。在此，我们使用全转录组测序技术，系统地研究了绒山羊胚胎皮肤样本中由环状RNA（circRNAs）、微小RNA（miRNAs）和信使RNA（mRNAs）介导的竞争性内源RNA（ceRNA）调控网络。我们分别获得了6468个、394个和239个显著差异表达的mRNA、circRNA和miRNA。这些鉴定出的RNA被进一步用于构建绒山羊毛囊发育后期由circRNAs介导的ceRNA调控网络。在鉴定出的分子种类中，miR-184和成纤维细胞生长因子（FGF）10表现出竞争性靶向相互作用。在次级毛囊真皮乳头细胞（SHF-DPCs）中，miR-184通过竞争性释放FGF10促进细胞增殖、抑制细胞凋亡并改变细胞周期。本研究报道FGF10及其与ncRNAs的相互作用显著影响SHF-DPCs，为绒山羊和其他哺乳动物毛囊生物学研究提供了参考。
