Meng Xiangyong, Shen Qinqin, Song Teng, Zhao Honglei, Zhang Yong, Ren Aiqing, Yang Wenbin
Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, Key Laboratory of Biomedicine in Gene Diseases and Health of Anhui Higher Education Institutes, College of Life Sciences, Anhui Normal University, Wuhu 241000, China.
School of Ecology and Environment, Anhui Normal University, Wuhu 241000, China.
Foods. 2023 Jul 5;12(13):2602. doi: 10.3390/foods12132602.
In order to develop a reliable and rapid method for meat freshness detection, nanocellulose (TOCNF) prepared via the TEMPO (2,2,6,6-tetramethylpiperidine oxidation) oxidation method was used as raw material to prepare hydrogels using Zn coordination and binding. Physicochemical properties such as water absorption and porosity were analyzed. It was further used to select suitable hydrogels for the preparation of indication labels after anthocyanin adsorption, and it was applied in the freshness detection of fresh minced pork. Five percent TOCNF (/) aqueous solution was homogenized by high shear for 4 min, and 20% (/) zinc chloride solution was added to it, so that the concentration of zinc ions could reach 0.25 mol/L. After standing for 24 h, the hydrogel was obtained with good water absorption and a porous three-dimensional network structure. The activation energies of volatile base nitrogen (TVBN) and anthocyanin indicating label color changes were 59.231 kJ/mol and 69.453 kJ/mol, respectively. The difference between the two is within 25 kJ/mol, so the prepared indicator label can accurately visualize the shelf life of fresh pork.
为开发一种可靠、快速的肉类新鲜度检测方法，以通过TEMPO（2,2,6,6-四甲基哌啶氧化）氧化法制备的纳米纤维素（TOCNF）为原料，利用锌配位结合制备水凝胶。分析了其吸水和孔隙率等理化性质。进一步用于筛选花青素吸附后适合制备指示标签的水凝胶，并将其应用于新鲜猪肉糜的新鲜度检测。将5%（/）的TOCNF水溶液通过高剪切均质4分钟，然后加入20%（/）的氯化锌溶液，使锌离子浓度达到0.25mol/L。静置24小时后，得到具有良好吸水性和多孔三维网络结构的水凝胶。挥发性盐基氮（TVBN）和花青素指示标签颜色变化的活化能分别为59.231kJ/mol和69.453kJ/mol。两者差值在25kJ/mol以内，因此制备的指示标签能够准确直观地反映新鲜猪肉的货架期。
