Chemistry Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
Chemistry Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
Int J Biol Macromol. 2020 Nov 15;163:649-656. doi: 10.1016/j.ijbiomac.2020.07.029. Epub 2020 Jul 6.
Zinc crosslinked supramolecular hydrogels from O-carboxymethyl chitosan biguanidine were successfully prepared. The structures of the prepared hydrogels were verified by H NMR, FTIR and XRD. FE-SEM verified the porous structure of hydrogels especially at high contents of Zn ions. The integral procedure decomposition temperatures calculated from TGA curves showed that the thermal stability was increased upon increasing the content of Zn ions. Cytotoxicity assays confirmed that the amounts of Zn ions released from hydrogels were below the toxic level towards a breast cancer cell line (MCF-7). The prepared hydrogels exhibited a significant microbial inhibition towards different species of bacteria and fungi, particularly at high Zn contents. Based on the obtained interesting data, the prepared zinc/O-carboxymethyl chitosan biguanidine could be used in drug delivery and biomedical applications.
成功制备了由 O-羧甲基壳聚糖盐酸胍交联的超分子水凝胶。通过 H NMR、FTIR 和 XRD 验证了水凝胶的结构。FE-SEM 验证了水凝胶的多孔结构，特别是在高浓度 Zn 离子时。从 TGA 曲线计算的积分程序分解温度表明，随着 Zn 离子含量的增加，热稳定性增加。细胞毒性试验证实，水凝胶中释放的 Zn 离子量低于对乳腺癌细胞系（MCF-7）的毒性水平。所制备的水凝胶对不同种类的细菌和真菌表现出显著的抑制作用，尤其是在高 Zn 含量时。基于获得的有趣数据，制备的锌/O-羧甲基壳聚糖盐酸胍可用于药物输送和生物医学应用。
