针对高分子量抗原的自身抗体的等电聚焦和反向免疫印迹法
Isoelectric focusing and reverse immunoblotting of autoantibodies against high molecular weight antigens.
作者信息
Stott D I, McLearie J
出版信息
Immunol Invest. 1986 Apr;15(2):113-22. doi: 10.3109/08820138609094137.
PMID:3744441
Abstract
A method for the clonal analysis of antibodies against high M.Wt. antigens is described. It involves isoelectric focusing, electrophoretic transfer of the focused antibodies to a nitrocellulose membrane and detection of the membrane-bound antibodies by overlay with radiolabelled antigen (reverse immunoblotting). The application of this technique to the clonal analysis of autoantibodies against thyroglobulin and DNA is described.
摘要
描述了一种针对高分子量抗原的抗体克隆分析方法。该方法包括等电聚焦、将聚焦后的抗体电泳转移至硝酸纤维素膜上，以及通过与放射性标记抗原重叠来检测膜结合抗体（反向免疫印迹法）。还描述了该技术在针对甲状腺球蛋白和DNA的自身抗体克隆分析中的应用。
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