Department of Agriculture, Ecotrophology, and Landscape Development, Anhalt University of Applied Sciences, 06406 Bernburg, Germany.
Institute of Crop Sciences, University of Hohenheim, 70593 Stuttgart, Germany.
Int J Mol Sci. 2023 Jun 22;24(13):10489. doi: 10.3390/ijms241310489.
Priming plants with beneficial microbes can establish rapid and robust resistance against numerous pathogens. Here, compelling evidence is provided that the treatment of rapeseed plants with OMG16 and FZB42 induces defence activation against infection. The relative expressions of the JA biosynthesis genes and , the ET biosynthesis genes and and the SA biosynthesis and signalling genes and were analysed separately in leaf, stem and root tissues using qRT-PCR. To successfully colonize rapeseed roots, the strain 43 pathogen suppressed the biosynthesis of JA, ET and SA hormones in non-primed plants. Priming led to fast and strong systemic responses of JA, ET and SA biosynthesis and signalling gene expression in each leaf, stem and root tissue. Moreover, the quantification of plant hormones via UHPLC-MS analysis revealed a 1.7- and 2.6-fold increase in endogenous JA and SA in shoots of primed plants, respectively. In roots, endogenous JA and SA levels increased up to 3.9- and 2.3-fold in Vl43-infected primed plants compared to non-primed plants, respectively. Taken together, these data indicate that microbial priming stimulates rapeseed defence responses against infection and presumably transduces defence signals from the root to the upper parts of the plant via phytohormone signalling.
用有益微生物对植物进行预处理可以快速、有效地增强植物对多种病原体的抗性。本研究提供了令人信服的证据，表明用 OMG16 和 FZB42 处理油菜植物可以诱导对 的防御激活。使用 qRT-PCR 分别在叶片、茎和根组织中分析了 JA 生物合成基因 和 、ET 生物合成基因 和 以及 SA 生物合成和信号转导基因 和 的相对表达。为了成功定殖油菜根，病原菌 43 株抑制了未预处理植物中 JA、ET 和 SA 激素的生物合成。预处理导致 JA、ET 和 SA 生物合成和信号转导基因表达在每个叶片、茎和根组织中快速而强烈的系统性反应。此外，通过 UHPLC-MS 分析定量植物激素显示，预处理植物的芽中内源 JA 和 SA 分别增加了 1.7 倍和 2.6 倍。在根中，与未预处理植物相比，Vl43 感染的预处理植物中内源 JA 和 SA 水平分别增加了 3.9 倍和 2.3 倍。总之，这些数据表明，微生物预处理可以刺激油菜对 的防御反应，并通过植物激素信号从根部向植物的上部传递防御信号。
