Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease, Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2023 Jun 25;24(13):10598. doi: 10.3390/ijms241310598.
Antimicrobial peptides (AMPs) are naturally occurring molecules found in various organisms that can help to defend against invading microorganisms and reduce the likelihood of drug resistance development. This study focused on the isolation of new AMPs from the genome library of a Gram-positive bacterium called sp. H5. To achieve this, we used the expression system and employed bioinformatics techniques to optimize and modify the peptides, resulting in the development of a new synthetic antimicrobial peptide (SAMP). Ap920 is expected to be a new antimicrobial peptide with a high positive charge (+12.5). Through optimization, a new synthetic antimicrobial peptide, Ap920-WI, containing only 15 amino acids, was created. Thereafter, the antimicrobial and antifungal activities of Ap920-WI were determined using minimum inhibitory concentration (MIC) and the concentration for 50% of maximal effect (EC). The Ap920-WI peptide was observed to target the outer membrane of fungal hyphae, leading to inhibition of growth in , , and . In plants, Ap920-WI showed significant antifungal activity and inhibited the infestation of on rape leaves. Importantly, Ap920-WI was found to be safe for mammalian cells since it did not show any hemolytic activity against sheep red blood cells. Overall, the study found that the new synthetic antimicrobial peptide Ap920-WI exhibits broad-spectrum activity against microorganisms and may offer a new solution for controlling plant diseases, as well as hold potential for drug development.
抗菌肽（AMPs）是存在于各种生物体中的天然分子，可以帮助抵御入侵的微生物，并降低耐药性发展的可能性。本研究专注于从革兰氏阳性菌 sp. H5 的基因组文库中分离新的 AMP。为此，我们使用了 表达系统，并运用生物信息学技术对肽进行优化和修饰，从而开发出一种新的合成抗菌肽（SAMP）。Ap920 预计是一种带有高正电荷（+12.5）的新型抗菌肽。通过优化，开发出了一种新的合成抗菌肽 Ap920-WI，仅包含 15 个氨基酸。此后，通过最小抑菌浓度（MIC）和 50%最大效应浓度（EC）来确定 Ap920-WI 的抗菌和抗真菌活性。Ap920-WI 肽靶向真菌菌丝的外膜，导致在 、 和 中生长受到抑制。在植物中，Ap920-WI 表现出显著的抗真菌活性，并抑制了 对油菜叶片的侵害。重要的是，Ap920-WI 对哺乳动物细胞是安全的，因为它对绵羊红细胞没有任何溶血活性。总的来说，该研究发现，新的合成抗菌肽 Ap920-WI 对微生物具有广谱活性，可能为控制植物病害提供新的解决方案，并有可能用于药物开发。
