Ouaissi M A, Neyrinck J L, Capron A
Int Arch Allergy Appl Immunol. 1986;81(1):75-80. doi: 10.1159/000234111.
A competitive radioimmunoassay (CRIA) for quantitating human plasma fibronectin levels has been developed and compared with a conventional immunoturbidimetry assay (IMTA). The assay ranges for CRIA and IMTA were 0.05-5 micrograms/ml and 100-1,000 micrograms/ml, respectively, and the lowest detectable amounts of fibronectin that differed significantly from zero were 17.6 ng and 50 micrograms. The correlation coefficient between CRIA and IMTA was r = 0.953 (p less than 0.001). We consider the CRIA as potentially useful in the identification and study of fibronectin in certain biological fluids where it may be present in low concentrations.
已开发出一种用于定量检测人血浆纤连蛋白水平的竞争性放射免疫分析（CRIA），并与传统免疫比浊法分析（IMTA）进行了比较。CRIA和IMTA的检测范围分别为0.05 - 5微克/毫升和100 - 1000微克/毫升，与零有显著差异的纤连蛋白最低检测量分别为17.6纳克和50微克。CRIA与IMTA之间的相关系数为r = 0.953（p < 0.001）。我们认为CRIA在某些可能含有低浓度纤连蛋白的生物体液中纤连蛋白的鉴定和研究方面可能有用。
