Wallaert B, Bart F, Aerts C, Ouaissi A, Hatron P Y, Tonnel A B, Voisin C
Départment de Pneumologie, Hôpital A Calmette, Lille, France.
Thorax. 1988 Jan;43(1):24-30. doi: 10.1136/thx.43.1.24.
A study was initiated to determine whether alveolar macrophages from patients with collagen vascular diseases but free of pulmonary symptoms were spontaneously activated and whether they released various mediators related to the pathogenesis of pulmonary fibrosis. Alveolar macrophages obtained by bronchoalveolar lavage from 32 patients with proved collagen vascular disease but no evidence of lung disease were compared with those from 10 patients with collagen vascular disease with interstitial lung disease (CVD-ILD) and from 10 healthy controls. The total number of alveolar macrophages did not differ between patients with collagen vascular disease and controls but were substantially increased in the CVD-ILD group. Alveolar macrophages from 31 of the 32 patients with collagen vascular disease and from all 10 in the CVD-ILD group had at least one criterion of activation. Neutrophil chemotactic activity was detected in supernatants from alveolar macrophage culture in 23 of the 32 patients with collagen vascular disease and from nine of the 10 in the CVD-ILD group; fibronectin secretion by alveolar macrophages was increased in 12 of the 32 patients with collagen vascular disease and in nine of the 10 in the CVD-ILD group. Furthermore, alveolar macrophages from 20 of the 32 patients with collagen vascular disease and four of the 10 CVD-ILD patients spontaneously released increased amounts of superoxide anion. Thus alveolar macrophages were spontaneously activated in a high proportion of patients with collagen vascular disease.
开展了一项研究，以确定无肺部症状的胶原血管病患者的肺泡巨噬细胞是否被自发激活，以及它们是否释放与肺纤维化发病机制相关的各种介质。将通过支气管肺泡灌洗从32例已证实患有胶原血管病但无肺部疾病证据的患者中获取的肺泡巨噬细胞，与从10例患有胶原血管病合并间质性肺病（CVD-ILD）的患者以及10名健康对照者中获取的肺泡巨噬细胞进行比较。胶原血管病患者和对照组的肺泡巨噬细胞总数没有差异，但在CVD-ILD组中显著增加。32例胶原血管病患者中的31例以及CVD-ILD组的所有10例患者的肺泡巨噬细胞至少有一项激活标准。在32例胶原血管病患者中的23例以及CVD-ILD组的10例中的9例的肺泡巨噬细胞培养上清液中检测到中性粒细胞趋化活性；32例胶原血管病患者中的12例以及CVD-ILD组的10例中的9例的肺泡巨噬细胞纤连蛋白分泌增加。此外，32例胶原血管病患者中的20例以及10例CVD-ILD患者中的4例的肺泡巨噬细胞自发释放的超氧阴离子量增加。因此，在很大比例的胶原血管病患者中，肺泡巨噬细胞被自发激活。
