Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
Key Laboratory of Traceability for Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China.
Int J Mol Sci. 2023 Jun 26;24(13):10641. doi: 10.3390/ijms241310641.
is the first identified SIP1 (6b Interacting Protein1) subfamily gene of the trihelix transcription factor family from We previously used a reverse genetic method to reveal its abiotic stress response function in endowing plants resistance to drought and salinity, as well as ABA (Abscisic acid). However, the molecular mechanisms of are unclear. In this study, the global transcriptome files of -overexpressing transgenic and wildtype seedlings under ABA treatment were constructed using RNA-seq. A total of 1823 and 5512 DEGs (Differentially Expressed Genes) were identified in OE vs. WT and OE_ABA vs. WT_ABA comparison groups, which included 751 and 2567 up-regulated DEGs, and 1072 and 2945 down-regulated DEGs, separately. The impact of overexpressed on plants was amplified by ABA, indicating was an ABA-conditioned responsive gene. More interestingly, we found the reasons for increasing plants' insensitivity to ABA were not by regulating ABA synthesis and catabolism, but by manipulating ABA transportation, ABA signal perception and transduction, inositol phosphate metabolism, as well as endomembrane trafficking, indirectly suggesting this gene may play roles upstream of the core ABA response pathway. Our results provided new insights into improving the knowledge about the function of and the ABA signaling mechanism in
是从三螺旋转录因子家族中鉴定出的 SIP1（6b 相互作用蛋白 1）亚家族基因，我们之前使用反向遗传学方法揭示了其在赋予植物抗旱和耐盐以及 ABA（脱落酸）方面的非生物胁迫响应功能。然而，的分子机制尚不清楚。在这项研究中，我们使用 RNA-seq 构建了 ABA 处理下过表达转基因和野生型幼苗的全转录组文件。OE vs. WT 和 OE_ABA vs. WT_ABA 比较组中分别鉴定出 1823 和 5512 个 DEGs（差异表达基因），其中包括 751 和 2567 个上调 DEGs，以及 1072 和 2945 个下调 DEGs。ABA 放大了过表达对植物的影响，表明是一种 ABA 条件响应基因。更有趣的是，我们发现过表达增加植物对 ABA 不敏感性的原因不是通过调节 ABA 的合成和分解代谢，而是通过操纵 ABA 的运输、ABA 信号感知和转导、肌醇磷酸盐代谢以及内膜运输，间接表明该基因可能在 ABA 核心响应途径的上游发挥作用。我们的研究结果为提高对基因功能的认识提供了新的见解，以及 ABA 信号转导机制在
