School of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan, 411201, China.
Yuelushan Laboratory, Changsha, 410125, China.
BMC Plant Biol. 2024 Apr 5;24(1):245. doi: 10.1186/s12870-024-04918-8.
Seed germination is an important development process in plant growth. The phytohormone abscisic acid (ABA) plays a critical role during seed germination. However, the mechanism of rapeseed in response to ABA is still elusive. In order to understand changes of rapeseed under exogenous ABA treatment, we explored differentially expressed metabolites (DEMs) and the differentially expressed genes (DEGs) between mock- and ABA-treated seedlings. A widely targeted LC-MS/MS based metabolomics were used to identify and quantify metabolic changes in response to ABA during seed germination, and a total of 186 significantly DEMs were identified. There are many compounds which are involved in ABA stimuli, especially some specific ABA transportation-related metabolites such as starches and lipids were screened out. Meanwhile, a total of 4440 significantly DEGs were identified by transcriptomic analyses. There was a significant enrichment of DEGs related to phenylpropanoid and cell wall organization. It suggests that exogenous ABA mainly affects seed germination by regulating cell wall loosening. Finally, the correlation analysis of the key DEMs and DEGs indicates that many DEGs play a direct or indirect regulatory role in DEMs metabolism. The integrative analysis between DEGs and DEMs suggests that the starch and sucrose pathways were the key pathway in ABA responses. The two metabolites from starch and sucrose pathways, levan and cellobiose, both were found significantly down-regulated in ABA-treated seedlings. These comprehensive metabolic and transcript analyses provide useful information for the subsequent post-transcriptional modification and post germination growth of rapeseed in response to ABA signals and stresses.
种子萌发是植物生长过程中的一个重要发育过程。植物激素脱落酸（ABA）在种子萌发过程中起着关键作用。然而，油菜种子对 ABA 的反应机制仍不清楚。为了了解外源 ABA 处理下油菜种子的变化，我们探索了模拟和 ABA 处理的幼苗之间差异表达的代谢物（DEMs）和差异表达的基因（DEGs）。我们采用广泛靶向的 LC-MS/MS 代谢组学方法来鉴定和定量分析种子萌发过程中 ABA 刺激下的代谢变化，共鉴定出 186 个显著的 DEMs。其中有许多化合物参与了 ABA 刺激，特别是筛选出了一些特定的与 ABA 运输相关的代谢物，如淀粉和脂质。同时，通过转录组分析鉴定出 4440 个显著的 DEGs。与苯丙烷和细胞壁组织相关的 DEGs 有明显富集。这表明外源 ABA 主要通过调节细胞壁松弛来影响种子萌发。最后，关键 DEMs 和 DEGs 的相关性分析表明，许多 DEGs 对 DEMs 代谢起着直接或间接的调节作用。DEGs 和 DEMs 的综合分析表明，淀粉和蔗糖途径是 ABA 反应的关键途径。淀粉和蔗糖途径中的两种代谢物，果聚糖和纤维二糖，在 ABA 处理的幼苗中均发现显著下调。这些综合的代谢和转录分析为油菜种子在响应 ABA 信号和胁迫后进行后续的转录后修饰和萌发后生长提供了有用的信息。
