Department of Chemistry and Chemical Engineering, 4 Mamaia Blvd., Ovidius University of Constanta, 900527 Constanta, Romania.
Department of Natural Sciences, Faculty of Natural and Agricultural Sciences 1, Ovidius University of Constanta, University Street, 900527 Constanta, Romania.
Molecules. 2023 Jun 22;28(13):4920. doi: 10.3390/molecules28134920.
Black poplar buds have high contents of many compounds with therapeutic potential, which are useful in cosmetics and the treatment of various dermatitis, respiratory diseases, etc. The aim of this study was to identify and exploit the local plant resources with biologically active properties from the Dobrogea area, Romania. For this purpose, materials were collected from the mentioned area, and macerates of black poplar were prepared in order to evaluate their qualities as antioxidant and antimicrobial agents. Three different black poplar buds' hydroalcoholic macerates were analyzed by the Folin-Ciocâlteau method to estimate the total content of phenolic compounds, by the HPLC-DAD method for identification and quantification of the main bioactive compounds and by the DPPH radical scavenging method to evaluate the antioxidant activity. All hydroalcoholic macerates showed high concentrations of phenolic compounds, the main individual compounds being gallic acid, chlorogenic acid, cinnamic acid, and methyl gallic acid. The antioxidant activity of the black poplar buds' hydroalcoholic macerates, evaluated by the DPPH radical scavenging test, showed high values, between 496 and 1200 mg GAE /100 g d.w. The Cd, Cu, Zn, Ni, and Pb concentrations released in dry poplar buds, determined by AAS, were below the detection limits. Hydroalcoholic macerates of black poplar were tested against two groups of gram-positive bacteria ( and ) using an agar well diffusion assay. The in vitro inhibitory activities of the macerates were important and ranged from 8.2-9.4 mm inhibition zones ( to 8.6 -10 mm inhibition zones ().
黑柳树芽含有许多具有治疗潜力的化合物，可用于化妆品和治疗各种皮炎、呼吸道疾病等。本研究旨在从罗马尼亚多布罗加地区鉴定和开发具有生物活性的本地植物资源。为此，从该地区采集了材料，并制备了黑柳树芽的浸膏，以评估其作为抗氧化剂和抗菌剂的特性。用 Folin-Ciocâlteau 法分析了三种不同的黑柳树芽水醇浸膏，以评估总酚含量；用 HPLC-DAD 法鉴定和定量分析主要生物活性化合物；用 DPPH 自由基清除法评估抗氧化活性。所有水醇浸膏均显示出高浓度的酚类化合物，主要的单体化合物为没食子酸、绿原酸、肉桂酸和甲基没食子酸。用 DPPH 自由基清除试验评估的黑柳树芽水醇浸膏的抗氧化活性表现出高值，介于 496 至 1200 mg GAE/100 g d.w.之间。通过 AAS 测定的干柳树芽中 Cd、Cu、Zn、Ni 和 Pb 浓度均低于检测限。采用琼脂孔扩散法测试了黑柳树芽水醇浸膏对两组革兰氏阳性菌（ 和 ）的抑制活性。浸膏的体外抑制活性较强，抑菌圈直径为 8.2-9.4mm（ ）至 8.6-10mm（）。
