Sahlabgi Alaa, Lupuliasa Dumitru, Stanciu Gabriela, Lupșor Simona, Vlaia Lavinia Lia, Rotariu Ramona, Predescu Nicoleta Corina, Rădulescu Cristiana, Olteanu Radu-Lucian, Stănescu Sorina-Geanina, Hîncu Lucian, Mititelu Magdalena
Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, "Carol Davila" University of Medicine and Pharmacy, 020945 Bucharest, Romania.
Department of Chemistry and Chemical Engineering, Ovidius University of Constanta, 900527 Constanta, Romania.
Gels. 2025 Feb 20;11(3):149. doi: 10.3390/gels11030149.
This study investigates the development and comparative evaluation of new dermatocosmetic preparations based on hydroalcoholic macerates of rosemary ( L.), focusing on their antioxidant, antimicrobial, and anti-inflammatory properties. For this purpose, rosemary hydroalcoholic macerations were analyzed by evaluating the content of biologically active compounds, determining their antioxidant and antimicrobial capacity. Total polyphenol content (TPC), determined via the Folin-Ciocâlteu method, reached 2155 ± 2.45 mg GAE/100 g fresh weight in the 70% ethanol macerate (RDS2) of rosemary from Dobrogea, significantly exceeding ( < 0.05) the values observed in the Bulgarian samples. The highest antioxidant activity (745 ± 2.33 mg GAE/100 g fresh weight) correlated with this extraction. Atomic absorption spectroscopy (AAS) analysis revealed elevated calcium (119.5 mg/kg), zinc, and iron levels in Dobrogean rosemary compared to its Bulgarian counterparts. Antimicrobial assessments demonstrated that the 70% ethanol macerate (RDS2) of Dobrogean rosemary exhibited the strongest inhibitory effects, particularly against (inhibition zone: 11-23 mm), while its activity against was moderate (10-17 mm at 30 µL). was also significantly inhibited, with an inhibition zone of 9-20 mm. In contrast, the Bulgarian rosemary macerate (RBS2) exhibited weak inhibition against the tested microorganisms. The higher antimicrobial activity of the RDS2 is likely due to its enriched polyphenolic content, including carnosic acid and rosmarinic acid, which are known for their bioactive properties. These findings highlight Dobrogean rosemary's superior bioactive properties, supporting its use in formulations with antioxidant and antimicrobial benefits.
本研究调查了基于迷迭香叶水醇浸液的新型皮肤化妆品制剂的开发及比较评估，重点关注其抗氧化、抗菌和抗炎特性。为此，通过评估生物活性化合物的含量、测定其抗氧化和抗菌能力，对迷迭香叶水醇浸液进行了分析。采用福林-酚法测定的总多酚含量（TPC）显示，来自多布罗加地区的迷迭香叶在70%乙醇浸液（RDS2）中达到2155±2.45毫克没食子酸当量/100克鲜重，显著超过（<0.05）保加利亚样品中的数值。与此提取物相关的抗氧化活性最高（745±2.33毫克没食子酸当量/100克鲜重）。原子吸收光谱（AAS）分析表明，与保加利亚的迷迭香叶相比，多布罗加地区的迷迭香叶中钙（119.5毫克/千克）、锌和铁的含量更高。抗菌评估表明，多布罗加地区迷迭香叶的70%乙醇浸液（RDS2）表现出最强的抑制作用，尤其是对金黄色葡萄球菌（抑菌圈：11 - 23毫米）；而其对大肠杆菌的活性为中等（30微升时抑菌圈为10 - 17毫米）。枯草芽孢杆菌也受到显著抑制，抑菌圈为9 - 20毫米。相比之下，保加利亚迷迭香叶浸液（RBS2）对测试微生物的抑制作用较弱。RDS2较高的抗菌活性可能归因于其丰富的多酚含量，包括具有生物活性特性的鼠尾草酸和迷迭香酸。这些发现突出了多布罗加地区迷迭香叶卓越的生物活性特性，支持其在具有抗氧化和抗菌功效的制剂中的应用。
