Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, Guanajuato 36050, Mexico.
Departamento de Genética Molecular, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Circuito Exterior s/n, Mexico City 04510, Mexico.
Molecules. 2023 Jun 28;28(13):5042. doi: 10.3390/molecules28135042.
The signal transduction paradigm in bacteria involves two-component systems (TCSs). are archaea that may have originated the current eukaryotic lifeforms. Most research on these archaea has focused on eukaryotic-like features, such as genes involved in phagocytosis, cytoskeleton structure, and vesicle trafficking. However, little attention has been given to specific prokaryotic features. Here, the sequence and predicted structural features of TCS sensor kinases analyzed from two metagenome assemblies and a genomic assembly from cultured Asgardian archaea are presented. The homology of the sensor kinases suggests the grouping closer to bacterial homologs. In contrast, one group from a and a meta-genome assembly from suggest the presence of a set of kinases separated from the typical bacterial TCS sensor kinases. AtoS and ArcB homologs were found in meta-genome assemblies along with defined domains for other well-characterized sensor kinases, suggesting the close link between these organisms and bacteria that may have resulted in the metabolic link to the establishment of symbiosis. Several kinases are predicted to be cytoplasmic; some contain several PAS domains. The data shown here suggest that TCS kinases in Asgardian bacteria are witnesses to the transition from bacteria to eukaryotic organisms.
细菌中的信号转导范例涉及双组分系统（TCS）。古菌可能是当前真核生命形式的起源。对这些古菌的大多数研究都集中在真核生物样特征上，例如参与吞噬作用、细胞骨架结构和囊泡运输的基因。然而，很少关注特定的原核特征。本文介绍了从两个宏基因组组装体和培养的古菌基因组组装体中分析的 TCS 传感器激酶的序列和预测结构特征。传感器激酶的同源性表明它们与细菌同源物的分组更为接近。相比之下，来自 和 的宏基因组组装体中的一组则表明存在一组与典型细菌 TCS 传感器激酶分离的激酶。在宏基因组组装体中发现了 AtoS 和 ArcB 同源物，以及其他经过充分研究的传感器激酶的定义结构域，这表明这些生物体与细菌之间存在密切联系，这种联系可能导致了与共生建立的代谢联系。一些激酶被预测为细胞质内的；一些包含几个 PAS 结构域。这里显示的数据表明，古菌中的 TCS 激酶是从细菌到真核生物过渡的见证者。
