Cheng Guanghao, Shu Xiaochun, Wang Zhong, Wang Ning, Zhang Fengjiao
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.
Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Nanjing 210014, China.
Plants (Basel). 2023 Jun 27;12(13):2458. doi: 10.3390/plants12132458.
is an important plant with both medicinal and ornamental values. However, it does not have an efficient genetic transformation system, which makes it difficult to study gene function of the genus. Virus-induced gene silencing (VIGS) is an effective technique for studying gene functions in plants. In this study, we develop an efficient virus-induced gene-silencing (VIGS) system using the leaf tip needle injection method. The widely used TRV vector is constructed, and the () and () genes are selected as visual indicators in the VIGS system. As a result, it is observed that leaves infected with TRV- and TRV- both show a yellowing phenotype (loss of green), and the chlorosis range of TRV- was larger and deeper than that of TRV-. qRT-PCR results show that the expression levels of and are significantly reduced, and the silencing efficiency of is higher than that of . These results indicate that the VIGS system of was preliminarily established, and is more suitable as a gene-silencing indicator. For the monocotyledonous plant leaves with a waxy surface, the leaf tip injection method greatly improves the infiltration efficiency. The newly established VIGS system will contribute to gene functional research in species.
是一种具有药用和观赏价值的重要植物。然而，它没有高效的遗传转化系统，这使得研究该属的基因功能变得困难。病毒诱导的基因沉默（VIGS）是研究植物基因功能的一种有效技术。在本研究中，我们使用叶尖针刺注射法开发了一种高效的病毒诱导基因沉默（VIGS）系统。构建了广泛使用的TRV载体，并选择了（）和（）基因作为VIGS系统中的视觉指标。结果观察到，感染TRV-和TRV-的叶片均表现出黄化表型（绿色丧失），且TRV-的黄化范围比TRV-更大、更深。qRT-PCR结果表明，（）和（）的表达水平显著降低，且（）的沉默效率高于（）。这些结果表明初步建立了（）的VIGS系统，且（）更适合作为基因沉默指标。对于具有蜡质表面的单子叶植物叶片，叶尖注射法大大提高了浸润效率。新建立的VIGS系统将有助于（）物种的基因功能研究。
