Establishing a Virus-Induced Gene Silencing System in .

is an important plant with both medicinal and ornamental values. However, it does not have an efficient genetic transformation system, which makes it difficult to study gene function of the genus. Virus-induced gene silencing (VIGS) is an effective technique for studying gene functions in plants. In this study, we develop an efficient virus-induced gene-silencing (VIGS) system using the leaf tip needle injection method. The widely used TRV vector is constructed, and the () and () genes are selected as visual indicators in the VIGS system. As a result, it is observed that leaves infected with TRV- and TRV- both show a yellowing phenotype (loss of green), and the chlorosis range of TRV- was larger and deeper than that of TRV-. qRT-PCR results show that the expression levels of and are significantly reduced, and the silencing efficiency of is higher than that of . These results indicate that the VIGS system of was preliminarily established, and is more suitable as a gene-silencing indicator. For the monocotyledonous plant leaves with a waxy surface, the leaf tip injection method greatly improves the infiltration efficiency. The newly established VIGS system will contribute to gene functional research in species.