Cheng Qin, Huang Shiying, Lin Lan, Zhong Qi, Huang Tao, He Haohua, Bian Jianmin
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China.
College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.
Plants (Basel). 2023 Jun 29;12(13):2496. doi: 10.3390/plants12132496.
The photosynthetic capacity of flag leaf plays a key role in grain yield in rice. Nevertheless, there are few studies on the heterosis of the rice flag leaf. Therefore, this study focuses on investigating the genetic basis of heterosis for flag leaf in the indica super hybrid rice combination WFYT025 in China using a high-throughput next-generation RNA-seq strategy. We analyzed the gene expression of flag leaf in different environments and different time periods between WFYT025 and its female parent. After obtaining the gene expression profile of the flag leaf, we further investigated the gene regulatory network. Weighted gene expression network analysis (WGCNA) was used to identify the co-expressed gene sets, and a total of 5000 highly expressed genes were divided into 24 co-expression groups. In CHT025, we found 13 WRKY family transcription factors in SDGs under the environment of early rice and 16 WRKY family genes in SDGs of under the environment of middle rice. We found nine identical transcription factors in the two stages. Except for five reported TFs, the other four TFs might play an important role in heterosis for grain number and photosynthesis. Transcription factors such as , and were found in both environments. To eliminate the influence of the environment, we examined the metabolic pathway with the same SDG (SSDG) in two environments. There were 312 SSDGs in total. These SSDGs mainly focused on the phosphorus metallic process, phosphorylation, plasma membrane, etc. These results provide resources for studying heterosis during super hybrid rice flag leaf development.
剑叶的光合能力对水稻产量起着关键作用。然而，关于水稻剑叶杂种优势的研究较少。因此，本研究采用高通量下一代RNA测序策略，重点研究中国籼型超级杂交水稻组合WFYT025剑叶杂种优势的遗传基础。我们分析了WFYT025及其母本在不同环境和不同时期剑叶的基因表达。在获得剑叶的基因表达谱后，我们进一步研究了基因调控网络。利用加权基因共表达网络分析（WGCNA）来识别共表达基因集，总共5000个高表达基因被分为24个共表达组。在CHT025中，我们在早稻环境下的SDGs中发现了13个WRKY家族转录因子，在中稻环境下的SDGs中发现了16个WRKY家族基因。我们在两个阶段发现了9个相同的转录因子。除了5个已报道的转录因子外，其他4个转录因子可能在粒数和光合作用的杂种优势中发挥重要作用。在两种环境中都发现了 、 和 等转录因子。为了消除环境的影响，我们研究了两种环境下具有相同SDG（SSDG）的代谢途径。总共有312个SSDG。这些SSDG主要集中在磷金属过程、磷酸化、质膜等方面。这些结果为研究超级杂交水稻剑叶发育过程中的杂种优势提供了资源。
