Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.
Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China.
Int J Mol Sci. 2022 Oct 26;23(21):12934. doi: 10.3390/ijms232112934.
Heterosis is a common biological phenomenon that is useful for breeding superior lines. Using heterosis to increase the yield and quality of crops is one of the main achievements of modern agricultural science. In this study, we analysed the transcriptome and metabolome of two three-line hybrid rice varieties, Taiyou 871 (TY871), and Taiyou 398 (TY398) and the parental grain endosperm using RNA-seq (three biological repeats per variety) and untargeted metabolomic (six biological repeats per variety) methods. TY871 and TY398 showed specific heterosis in yield and quality. Transcriptome analysis of the hybrids revealed 638 to 4059 differentially expressed genes in the grain when compared to the parents. Metabolome analysis of the hybrids revealed 657 to 3714 differential grain metabolites when compared to the parents. The honeydew1 and grey60 module core genes and are involved in the regulation of awn development, grain size, and grain number, as well as the regulation of grain length and plant height, respectively. Rice grain length may be an important indicator for improving the quality of three-line hybrid rice. In addition, the rice quality-related metabolite NEG_M341T662 was highly connected to the module core genes and . The functions of and are EF-hand calcium binding protein and late embroideries absolute protein repeat containing protein, respectively. These genes may play a role in the formation of rice quality. We constructed a gene and metabolite coexpression network, which provides a scientific basis for the utilization of heterosis in producing high-yield and high-quality hybrid rice.
杂种优势是一种常见的生物学现象，对于培育优良品系非常有用。利用杂种优势来提高作物的产量和品质是现代农业科学的主要成就之一。在这项研究中，我们使用 RNA-seq（每个品种三个生物学重复）和非靶向代谢组学（每个品种六个生物学重复）方法，分析了两个三系杂交水稻品种泰优 871（TY871）和泰优 398（TY398）及其亲本稻谷胚乳的转录组和代谢组。TY871 和 TY398 在产量和品质方面表现出特异性杂种优势。杂种的转录组分析显示，与亲本相比，在籽粒中有 638 到 4059 个差异表达基因。与亲本相比，杂种的代谢组分析显示，在籽粒中有 657 到 3714 个差异代谢物。蜜露 1 和灰色 60 模块核心基因和参与调控芒发育、粒长、粒数以及粒长和株高的调控。水稻粒长可能是提高三系杂交水稻品质的一个重要指标。此外，与模块核心基因和相关的水稻品质相关代谢物 NEG_M341T662 高度相连。和的功能分别是 EF 手钙离子结合蛋白和晚期绣花绝对蛋白重复包含蛋白。这些基因可能在水稻品质形成中发挥作用。我们构建了一个基因和代谢物的共表达网络，为利用杂种优势生产高产优质杂交水稻提供了科学依据。
