Alhammad Bushra Ahmed, Abdel-Aziz Heba M M, Seleiman Mahmoud F, Tourky Shaimaa M N
Biology Department, College of Science and Humanity Studies, Prince Sattam Bin Abdulaziz University, Al Kharj Box 292, Riyadh 11942, Saudi Arabia.
Botany Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt.
Plants (Basel). 2023 Jun 30;12(13):2509. doi: 10.3390/plants12132509.
Through interactions with plant cells, silver nanoparticles (AgNPs) with both biological and chemical origins can stimulate physiological and metabolic processes in plants. To ensure their safe application in the food chain, it is necessary to investigate their effects on plant systems. Therefore, the effects of chemical AgNPs (chem-AgNPs) and biologically synthesized AgNPs (bio-AgNPs) at different levels (i.e., 0, 10, and 50 ppm) on physiological and biochemical traits {i.e., root and shoot growth traits, photosynthetic pigments (Chl , Chl , carotenoids, and total pigments), soluble sugars, total carbohydrates, starch, HO, and antioxidant enzyme activities} of L. seedlings were investigated. AgNPs were biosynthesized from silver nitrate (AgNO) by a green synthesis approach using seed extract. The synthesized AgNPs were characterized by UV-vis spectroscopy, transmission electron microscopy (TEM), zeta potential, Fourier-transform infrared spectra (FT-IR), and X-ray diffraction (XRD). The results showed that bio-AgNPs at 10 ppm resulted in the highest growth, physiological, and biological traits of faba bean seedlings in comparison with those obtained from both AgNO and chem-AgNPs treatments. On the other hand, all AgNPs treatments adversely affected the chloroplast ultrastructure, however, fewer negative effects were obtained with the application of 10 ppm bio-AgNPs. In addition, the roots and shoots of seedlings contained the lowest Ag content under different treatments at 10 ppm AgNPs in comparison to the highest level of AgNPs (50 ppm), which indicates that additional studies should be incorporated to ensure safe use of lower concentrations of bio-AgNPs in seed priming. In conclusion, the application of biogenic nanoparticles at 10 ppm can be recommended to enhance plant growth and the productivity of strategic crops.
通过与植物细胞的相互作用，具有生物和化学来源的银纳米颗粒（AgNPs）可以刺激植物中的生理和代谢过程。为确保其在食物链中的安全应用，有必要研究它们对植物系统的影响。因此，研究了不同浓度（即0、10和50 ppm）的化学AgNPs（chem-AgNPs）和生物合成AgNPs（bio-AgNPs）对蚕豆幼苗生理生化特性{即根和地上部生长特性、光合色素（叶绿素a、叶绿素b、类胡萝卜素和总色素）、可溶性糖、总碳水化合物、淀粉、过氧化氢（H₂O₂）和抗氧化酶活性}的影响。采用绿豆种子提取物通过绿色合成方法从硝酸银（AgNO₃）生物合成AgNPs。通过紫外可见光谱、透射电子显微镜（TEM）、zeta电位、傅里叶变换红外光谱（FT-IR）和X射线衍射（XRD）对合成的AgNPs进行了表征。结果表明，与AgNO₃和chem-AgNPs处理相比，10 ppm的bio-AgNPs使蚕豆幼苗的生长、生理和生物学特性达到最高。另一方面，所有AgNPs处理均对叶绿体超微结构产生不利影响，然而，施用10 ppm的bio-AgNPs产生的负面影响较少。此外，与最高浓度的AgNPs（50 ppm）相比，在10 ppm AgNPs的不同处理下，幼苗的根和地上部含有的Ag含量最低，这表明应进行更多研究以确保在种子引发中安全使用较低浓度的bio-AgNPs。总之，建议施用10 ppm的生物源纳米颗粒以促进植物生长和战略作物的生产力。
