Behdarvandi Behrang, Goodwin Paul H
School of Environmental Sciences, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W, Canada.
Plants (Basel). 2023 Jul 4;12(13):2540. doi: 10.3390/plants12132540.
shows much higher mortality to root rot when grown in soil previously planted with ginseng than in soil not previously planted with ginseng, which is known as ginseng replant disease. Treatment of ginseng roots with methanol extracts of previous ginseng soils significantly increased root lesion sizes due to compared to roots treated with water or methanol extracts of ginseng roots or non-ginseng soils. Inoculation of water-treated roots with increased expression of a basic chitinase 1 gene (), neutral pathogenesis-related protein 5 gene () and pathogenesis-related protein 10-2 gene (), which are related to jasmonic acid (JA), ethylene (ET) or necrotrophic infection, and also increased expression of an acidic β-1-3-glucanase gene (), which is related to salicylic acid (SA). Infection did not affect expression of a cysteine protease inhibitor gene (). Following infection, roots treated with ginseng root extract mostly showed similar expression patterns as roots treated with water, but roots treated with previous ginseng soil extract showed reduced expression of , , and , but increased expression of . Methanol-soluble compound(s) in soil previously planted with ginseng are able to increase root lesion size, suppress JA/ET-related gene expression and trigger SA-related gene expression in ginseng roots during infection, and may be a factor contributing to ginseng replant disease.
与种植在未种植过人参的土壤中相比，种植在先前种植过人参的土壤中的人参对根腐病的死亡率要高得多，这就是所谓的人参连作障碍。用人参连作土壤的甲醇提取物处理人参根，与用水、人参根或非人参土壤的甲醇提取物处理的根相比，显著增加了根病斑大小。用 接种水处理过的根，与茉莉酸（JA）、乙烯（ET）或坏死性感染相关的碱性几丁质酶 1 基因（）、中性病程相关蛋白 5 基因（）和病程相关蛋白 10 - 2 基因（）的表达增加，同时与水杨酸（SA）相关的酸性β - 1,3 - 葡聚糖酶基因（）的表达也增加。感染不影响半胱氨酸蛋白酶抑制剂基因（）的表达。感染后，用人参根提取物处理的根大多表现出与水处理根相似的表达模式，但用连作人参土壤提取物处理的根显示 、 、 和 的表达降低，但 的表达增加。先前种植过人参的土壤中的甲醇溶性化合物能够在 感染期间增加人参根的病斑大小，抑制 JA/ET 相关基因的表达并触发 SA 相关基因的表达，可能是导致人参连作障碍的一个因素。
