Edwards J C, Sedgwick A D, Moore A R, Al-Duaij A Y, Willoughby D A
Int J Tissue React. 1986;8(4):309-19.
Observations have been made on histological changes in femoral-head articular cartilage transplanted into soft tissue in rats under a variety of conditions. Articular cartilage was implanted either whole or minced. Implants were made into deflated subcutaneous air pouches with or without an inflammatory irritant and into normal subcutaneous tissue. Cartilage-matrix loss occurred in four patterns: marginal zone encroachment, loss of metachromatic staining without loss of tissue mass, surface invasion and lacunar enlargement and coalescence. The presence of an inflammatory reaction had no discernible effect on loss of cartilage matrix. Changes at the marginal zone preceded changes at the centre. Loss of metachromatic staining without loss of tissue mass occurred in areas of chondrocyte death. Surface invasion was not affected by the viability of the underlying chondrocytes. Surface invasion occurred earliest in cartilage transplanted into subcutaneous tissue, and latest in cartilage implanted into air pouches inflamed with carrageenan. The onset of surface invasion appeared to be dependent on soft-tissue adhesion to the cartilage surface. Mincing of cartilage was associated with an increase in surface invasion in the deeper cartilage zone with large lacunae but not in the superficial zone with small lacunae. Occasional cut surfaces showed outgrowth of new cartilage matrix. These observations on artefactual modes of cartilage degradation may assist in analysing the critical steps in cartilage degradation in arthritic joints.
已对在多种条件下移植到大鼠软组织中的股骨头关节软骨的组织学变化进行了观察。关节软骨被完整植入或切碎后植入。植入物被植入已放气的皮下气囊，其中有的含有炎性刺激物，有的不含，同时也被植入正常皮下组织。软骨基质损失呈现出四种模式：边缘区侵蚀、异染性染色丧失但组织质量未丧失、表面侵入以及腔隙扩大和融合。炎症反应的存在对软骨基质损失没有明显影响。边缘区的变化先于中心区的变化。在软骨细胞死亡区域出现了异染性染色丧失但组织质量未丧失的情况。表面侵入不受其下方软骨细胞活力的影响。表面侵入最早出现在移植到皮下组织的软骨中，最晚出现在植入用角叉菜胶致炎的气囊中的软骨中。表面侵入的发生似乎取决于软组织与软骨表面的粘连。切碎软骨与较大腔隙的深层软骨区而非较小腔隙的浅层软骨区的表面侵入增加有关。偶尔可见新软骨基质从切面长出。这些关于软骨降解人为模式的观察结果可能有助于分析关节炎关节软骨降解的关键步骤。
