Galiyeva Aldana, Daribay Arailym, Zhumagaliyeva Tolkyn, Zhaparova Lyazzat, Sadyrbekov Daniyar, Tazhbayev Yerkeblan
Institute of Chemical Problems, Karagandy University of the Name of Academician E.A. Buketov, Karaganda City 100028, Kazakhstan.
Polymers (Basel). 2023 Jun 22;15(13):2774. doi: 10.3390/polym15132774.
The aim of this study was to create nanoparticles of human serum albumin immobilized with anti-TB drugs (rifampicin, isoniazid) using the desolvation method. Central Composite Design (CCD) was applied to study the effect of albumin, urea, L-cysteine, rifampicin and isoniazid concentration on particle size, polydispersity and loading degree of the drugs. The optimized nanoparticles were spherical in shape with an average particle size of 216.7 ± 3.7 nm and polydispersity of 0.286 ± 4.9. The loading degree of rifampicin and isoniazid in the optimized nanoparticles were 44% and 27%, respectively. The obtained nanoparticles were examined by Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC); the results showed the absence of drug-polymer interactions. The drug release from the polymer matrix was studied using dialysis membranes.
本研究的目的是采用去溶剂化法制备固定有抗结核药物（利福平、异烟肼）的人血清白蛋白纳米颗粒。应用中心复合设计（CCD）研究白蛋白、尿素、L-半胱氨酸、利福平和异烟肼浓度对粒径、多分散性和药物负载度的影响。优化后的纳米颗粒呈球形，平均粒径为216.7±3.7nm，多分散性为0.286±4.9。优化后的纳米颗粒中利福平和异烟肼的负载度分别为44%和27%。通过傅里叶变换红外光谱（FTIR）、热重分析（TGA）和差示扫描量热法（DSC）对所得纳米颗粒进行了检测；结果表明不存在药物-聚合物相互作用。使用透析膜研究了药物从聚合物基质中的释放。
