Quispe Mayte M, Villanueva María E, Copello Guillermo J, López Olivia V, Villar Marcelo A
Planta Piloto de Ingeniería Química, PLAPIQUI (UNS-CONICET), Camino La Carrindanga Km 7, Bahía Blanca 8000, Argentina.
Departamento de Ciencias Básicas, Universidad de Luján, Luján 6700, Argentina.
Polymers (Basel). 2023 Jun 30;15(13):2907. doi: 10.3390/polym15132907.
Poly(3-hydroxybutyrate), PHB, is a hydrophobic biopolymer with good mechanical and barrier properties. However, neat PHB is a semicrystalline polymer with a relative high degree of crystallinity and poor film properties. In this work, this biopolymer was plasticized with glycerol tributyrate and functionalized with copper (II) sulfate, allowing us to obtain biodegradable antimicrobial flexible films. Films with the minimum inhibitory concentration (MIC) of copper (II) sulfate presented a higher roughness than neat PHB films. The presence of plasticizer significantly improved the copper sulfate diffusion process, which was evidenced by a greater inhibition halo for plasticized materials compared to unplasticized ones, at the same salt concentration. Plasticized PHB with 2.5% copper (II) sulfate inhibited both Gram-positive () and Gram-negative () bacteria, as determined by the bacterial inhibition halo. In addition, neat PHB films and PHB containing copper (II) sulfate did not show in vitro cytotoxicity in the L-929 cell line. Thus, plasticized PHB functionalized with copper (II) sulfate can be used as biodegradable antimicrobial flexible films for different applications.
聚（3-羟基丁酸酯）（PHB）是一种具有良好机械性能和阻隔性能的疏水性生物聚合物。然而，纯PHB是一种半结晶聚合物，结晶度相对较高，薄膜性能较差。在本研究中，这种生物聚合物用三丁酸甘油酯增塑并用硫酸铜进行功能化处理，从而使我们能够获得可生物降解的抗菌柔性薄膜。硫酸铜最低抑菌浓度（MIC）的薄膜比纯PHB薄膜具有更高的粗糙度。增塑剂的存在显著改善了硫酸铜的扩散过程，这一点在相同盐浓度下，与未增塑材料相比，增塑材料的抑菌圈更大得到了证明。含2.5%硫酸铜的增塑PHB对革兰氏阳性菌和革兰氏阴性菌均有抑制作用，这通过细菌抑菌圈得以确定。此外，纯PHB薄膜和含硫酸铜的PHB薄膜在L-929细胞系中未表现出体外细胞毒性。因此，用硫酸铜功能化的增塑PHB可作为可生物降解的抗菌柔性薄膜用于不同应用。
