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番茄钙通透通道 4.1（SlOSCA4.1）是 Pepino mosaic virus 的易感性因子。

The tomato calcium-permeable channel 4.1 (SlOSCA4.1) is a susceptibility factor for pepino mosaic virus.

机构信息

Abiopep S.L., Parque Científico de Murcia, Murcia, Spain.

Centro de Edafología y Biología Aplicada del Segura (CEBAS)-CSIC, Campus Universitario de Espinardo, Murcia, Spain.

出版信息

Plant Biotechnol J. 2023 Oct;21(10):2140-2154. doi: 10.1111/pbi.14119. Epub 2023 Jul 13.

DOI:10.1111/pbi.14119
PMID:37448155
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10502756/
Abstract

The hyperosmolality-gated calcium permeable channel 4.1 (OSCA4.1) belongs to an evolutionarily conserved small family of mechano-sensitive channels. OSCA members may represent key players in plant resistance to drought and to pathogen infection but are scarcely studied. After screening for resistance to pepino mosaic virus (PepMV) a collection of 1000 mutagenized tomato families, we identified a mutant showing no symptoms and reduced virus accumulation. Resistance was mapped to chromosome 2 between positions 46 309 531 to 47 044 163, where a missense mutation caused the putative truncation of the OSCA4.1 protein. A CRISPR/Cas9 slosca4.1 mutant was resistant to PepMV, but not to tobacco mosaic virus or potato virus X. Inoculation of mutant and wild type tomato protoplasts showed that resistance was expressed in single cells, suggesting a role for SlOSCA4.1 in early viral function(s); congruently, SlOSCA4.1 re-localized to structures reminiscent of viral replication complexes. We propose that SlOSCA4.1 contributes to the correct regulation of the Ca homeostasis necessary for optimal PepMV infection. PepMV is a pandemic virus that causes significant losses in tomato crops worldwide. In spite of its importance, no tomato-resistant varieties have been deployed yet; the mutant identified here has great potential to breed tomato varieties resistant to PepMV.

摘要

超渗压门控钙通透通道 4.1（OSCA4.1）属于一个进化上保守的机械敏感通道小家族。OSCA 成员可能是植物抗旱和抗病原体感染的关键因素，但研究甚少。在筛选抗 Pepino 花叶病毒（PepMV）的 1000 个番茄诱变系后，我们鉴定到一个表现出无症和减少病毒积累的突变体。抗性被定位到染色体 2 上的 46309531 到 47044163 之间，其中一个错义突变导致推测的 OSCA4.1 蛋白截断。CRISPR/Cas9 slosca4.1 突变体对 PepMV 具有抗性，但对烟草花叶病毒或马铃薯 X 病毒没有抗性。突变体和野生型番茄原生质体的接种表明，抗性在单个细胞中表达，这表明 SlOSCA4.1 在早期病毒功能中发挥作用；一致地，SlOSCA4.1 重新定位于类似于病毒复制复合物的结构。我们提出，SlOSCA4.1 有助于正确调节钙稳态，这对最佳 PepMV 感染是必要的。PepMV 是一种世界性流行病毒，在全球范围内导致番茄作物的重大损失。尽管其重要性，但尚未部署任何抗番茄品种；这里鉴定的突变体具有培育抗 PepMV 番茄品种的巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/b6039b956b7f/PBI-21-2140-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/146e637d97b8/PBI-21-2140-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/9ef9574ade8e/PBI-21-2140-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/a7ea22a6e3a3/PBI-21-2140-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/236ff5d4ed74/PBI-21-2140-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/abfbd63522b3/PBI-21-2140-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/b6039b956b7f/PBI-21-2140-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/146e637d97b8/PBI-21-2140-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/9ef9574ade8e/PBI-21-2140-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/a7ea22a6e3a3/PBI-21-2140-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/236ff5d4ed74/PBI-21-2140-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/abfbd63522b3/PBI-21-2140-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3eae/11376745/b6039b956b7f/PBI-21-2140-g006.jpg

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