Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, Jilin, China.
Department of Orthopedics, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.
Front Endocrinol (Lausanne). 2023 Jun 23;14:1179521. doi: 10.3389/fendo.2023.1179521. eCollection 2023.
Evidence for a relationship between oxidative stress and osteoporotic fractures in humans is limited. Fluorescent oxidation products (FlOPs, excitation/emission wavelengths 320/420nm denoted FlOP_320; 360/420nm [FlOP_360]; and 400/475nm [FlOP_400]) are global biomarkers of oxidative stress, and reflect oxidative damage to proteins, phospholipids, and nucleic acids. We investigated the association between FlOPs and a recent osteoporotic fracture.
We conducted a case-control study in a Chinese population aged 50 years or older. A recent osteoporotic fracture in the cases was confirmed by x-ray. Cases were matched with community-based non-fracture controls (1:2 ratio) for age (± 4 years) and sex. In addition, we conducted a sensitivity unmatched case-control study which included all fracture cases and all eligible non-fracture controls prior to matching. Plasma FlOPs were measured with a fluorescent microplate reader. We used unconditional logistic regression to analyze the association between FlOPs (per 1-SD increase in logarithmic scale) and fracture; odds ratios (OR) and 95% confidence intervals (95% CI) were reported.
Forty-four cases and 88 matched controls (mean age: 68.2 years) were included. After covariate adjustment (i.e., body mass index, physical activity, and smoking), higher FlOP_360 (OR = 1.85; 95% CI = 1.03 - 3.34) and FlOP_400 (OR = 13.29; 95% CI = 3.48 - 50.69) levels, but not FlOP_320 (OR = 0.56; 95% CI = 0.27 - 1.15), were associated with increased fracture risk. Subgroup analyses by fracture site and unmatched case-control study found comparable associations of FlOP_360 and FlOP_400 with hip and non-hip fractures.
Higher FlOP_360 and FlOP_400 levels were associated with increased risk of fracture, and this association was comparable for hip and non-hip fractures. Prospective studies are warranted to confirm this finding.
人类氧化应激与骨质疏松性骨折之间关系的证据有限。荧光氧化产物（FlOPs，激发/发射波长 320/420nm 表示 FlOP_320；360/420nm [FlOP_360]；和 400/475nm [FlOP_400]）是氧化应激的全球生物标志物，反映了蛋白质、磷脂和核酸的氧化损伤。我们研究了 FlOPs 与最近的骨质疏松性骨折之间的关联。
我们在中国进行了一项 50 岁或以上人群的病例对照研究。病例通过 X 射线确认最近发生了骨质疏松性骨折。病例按年龄（± 4 岁）和性别与社区非骨折对照（1:2 比例）相匹配。此外，我们进行了一项未匹配的病例对照敏感性研究，该研究包括所有骨折病例和所有符合条件的非骨折对照。使用荧光微孔板读数器测量血浆 FlOPs。我们使用非条件逻辑回归分析 FlOPs（每增加 1-SD 对数）与骨折之间的关系；报告比值比（OR）和 95%置信区间（95%CI）。
纳入 44 例病例和 88 例匹配对照（平均年龄：68.2 岁）。调整协变量（即体重指数、体力活动和吸烟）后，较高的 FlOP_360（OR=1.85；95%CI=1.03-3.34）和 FlOP_400（OR=13.29；95%CI=3.48-50.69）水平与骨折风险增加相关，但 FlOP_320（OR=0.56；95%CI=0.27-1.15）水平与骨折风险增加无关。骨折部位的亚组分析和未匹配的病例对照研究发现，FlOP_360 和 FlOP_400 与髋部和非髋部骨折的相关性相似。
较高的 FlOP_360 和 FlOP_400 水平与骨折风险增加相关，且这种相关性在髋部和非髋部骨折中相似。需要前瞻性研究来证实这一发现。
