Mahmoud Ali Ibrahim, Mohamed Halby Hamada Mohamed, Abd-Elrady Bahaa-Eldin Anwar, Taha Salim Mohamed, Ahmed Mohamed Heba
Department of Microbiology and Immunology, Faculty of Pharmacy, Assiut Branch, Al-Azhar University, Assiut, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia, Egypt.
Iran J Microbiol. 2023 Jun;15(3):448-455. doi: 10.18502/ijm.v15i3.12906.
17 β-estradiol (E2) is an important pollutant of the aquatic system. It is responsible for sexual disruptions in the majority of aquatic organisms. This study aimed to search for bacteria with high potential degradation of E2 as an important method for bioremediation.
Sewage water samples were collected and treated to isolate bacterial strains which were identified by conventional methods and 16S ribosomal RNA gene sequence analysis. The biodegradation of E2 by the isolated strains was evaluated under different environmental conditions.
Two bacterial strains were recovered from sewage water samples and identified as and , (named ASc2 and ASc5 respectively). Co-culture of the two strains showed biodegradation of approximately 93.6 % of E2 (50 mg. L) within 48 hours. However, the biodegradation capacity of the same E2 concentration was 69.4% and 71.2% for ASc2 and ASc5 each alone, respectively. The optimum cultivation conditions for efficient E2 biodegradation by co-culture were 5% (v/v) inoculation volume with 50 mg. L of E2 as the initial concentration at pH 7 and 30°C within 48 hours inoculation period.
This study detected new bacterial strains that are capable of rapid degradation of estrogen as an environmental pollutant.
17β - 雌二醇（E2）是水生系统中的一种重要污染物。它是导致大多数水生生物性紊乱的原因。本研究旨在寻找具有高效降解E2潜力的细菌，作为生物修复的一种重要方法。
采集污水样本并进行处理，以分离细菌菌株，通过常规方法和16S核糖体RNA基因序列分析对其进行鉴定。在不同环境条件下评估分离菌株对E2的生物降解作用。
从污水样本中分离出两株细菌，分别鉴定为[具体菌名1]和[具体菌名2]（分别命名为ASc2和ASc5）。两株菌共培养时，在48小时内可使约93.6%的E2（50 mg·L）发生生物降解。然而，单独培养时，ASc2和ASc5对相同E2浓度的生物降解能力分别为69.4%和71.2%。共培养高效生物降解E2的最佳培养条件为接种量5%（v/v），初始浓度50 mg·L的E2，pH值为7，温度30°C，接种时间48小时。
本研究检测到能够快速降解作为环境污染物的雌激素的新细菌菌株。
