Nategh Fatemeh, Mohit Mitra, Saatian Minoo, Farahmand Zohreh, Soleimanjahi Hoorieh
Department of Public Medicine, School of Medicine, Tehran Medical Branch, Islamic Azad University, Tehran, Iran.
Department of Obstetrics and Gynecology, Tehran Medical Branch, Islamic Azad University, Tehran, Iran.
Iran J Microbiol. 2023 Jun;15(3):468-474. doi: 10.18502/ijm.v15i3.12909.
HPV infections cause a wide spectrum of pathological changes in lower anogenital epithelium. The aim of this study was to investigate the HPV DNA status and histological findings in cervical biopsy specimens diagnosed as flat condyloma.
This study included 20 cervical biopsy specimens diagnosed as flat condyloma. The histopathological criteria and presence of HPV DNA were evaluated. HPV genotyping was determined in HPV-positive specimens using BioEdit software and the results were analyzed in SPSS software.
HPV DNA was not found in 30% of specimens and relative frequency of HPV genotypes was: 15% HPV6, 15% HPV11, 5% HPV16, 5% HPV18, 5% HPV53, 5% HPV68, 5% HPV84, 10% HPV45. Relative frequency of histopathological criteria was as below: 100% of specimens had koilocytosis, 100% acanthosis, 15% nuclear immaturity, 100% atypia, 15% mitotic activity, 50% dyskeratosis, 35% parakeratosis and 10% hyperkeratosis.
There were significant differences between HPV positivity and two pathologic criteria; multinucleation and hyperkeratosis (P Value: 0.02). Nuclear immaturity was significantly more prevalent in high risk HPV-positive specimens (P Value: 0.03).
人乳头瘤病毒（HPV）感染会导致下肛门生殖器上皮出现广泛的病理变化。本研究的目的是调查诊断为扁平湿疣的宫颈活检标本中的HPV DNA状态和组织学发现。
本研究纳入了20例诊断为扁平湿疣的宫颈活检标本。评估了组织病理学标准和HPV DNA的存在情况。使用BioEdit软件对HPV阳性标本进行HPV基因分型，并在SPSS软件中分析结果。
30%的标本未检测到HPV DNA，HPV基因型的相对频率为：HPV6占15%，HPV11占15%，HPV16占5%，HPV18占5%，HPV53占5%，HPV68占5%，HPV84占5%，HPV45占10%。组织病理学标准的相对频率如下：100%的标本有挖空细胞，100%有棘层肥厚，15%有核未成熟，100%有异型性，15%有有丝分裂活性，50%有角化不良，35%有不全角化，10%有角化过度。
HPV阳性与两个病理标准（多核化和角化过度）之间存在显著差异（P值：0.02）。核未成熟在高危HPV阳性标本中明显更常见（P值：0.03）。
