Wang Zhongyan, Zhang Yueling, Aweya Jude Juventus, Lin Zhongyang, Yao Defu, Zheng Zhihong
Institute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China.
STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China.
Fish Shellfish Immunol Rep. 2023 Jun 26;5:100109. doi: 10.1016/j.fsirep.2023.100109. eCollection 2023 Dec 15.
LHPP (Phospholysine Phosphohistidine Inorganic Pyrophosphate Phosphatase) is a protein histidine phosphatase that modulates a hidden posttranslational modification called histidine phosphorylation. LHPP also acts as a tumor suppressor, which plays a pivotal role in various cellular processes. However, whether LHPP participates in the regulation of invertebrate's immunity is still unknown. Here we characterized a LHPP homolog in (designated LHPP), with a 807 bp length of open reading frame (ORF) encoding a putative protein of 268 amino acids. Sequence analysis revealed that LHPP contains a typical hydrolase 6 and hydrolase-like domain, which was conserved from invertebrate to vertebrate. LHPP was ubiquitously expressed in tissues and induced in hemocyte and hepatopancreas by and white spot syndrome virus (WSSV) challenge, indicating that LHPP participated in the immune responses. Moreover, silencing of LHPP followed by inhibited hemocyte apoptosis. This study enriches our current insight on shrimp immunity, and provides novel perspective to understand immune-regulatory role of LHPP.
LHPP（磷酸赖氨酸磷酸组氨酸无机焦磷酸磷酸酶）是一种蛋白质组氨酸磷酸酶，可调节一种名为组氨酸磷酸化的隐蔽翻译后修饰。LHPP还作为一种肿瘤抑制因子，在各种细胞过程中发挥关键作用。然而，LHPP是否参与无脊椎动物的免疫调节仍不清楚。在这里，我们鉴定了一种虾中的LHPP同源物（命名为LHPP），其开放阅读框（ORF）长度为807bp，编码一个推定的268个氨基酸的蛋白质。序列分析表明，LHPP含有一个典型的水解酶6和类水解酶结构域，从无脊椎动物到脊椎动物都保守。LHPP在组织中广泛表达，并在副溶血弧菌和白斑综合征病毒（WSSV）刺激下在血细胞和肝胰腺中被诱导，表明LHPP参与了免疫反应。此外，沉默LHPP后再用副溶血弧菌处理可抑制血细胞凋亡。本研究丰富了我们目前对虾免疫的认识，并为理解LHPP的免疫调节作用提供了新的视角。
