Japan Food Research Laboratories, 6-11-10 Nagayama, Tama-shi, Tokyo 206-0025, Japan.
J AOAC Int. 2023 Nov 2;106(6):1525-1531. doi: 10.1093/jaoacint/qsad083.
Edible insects may contain arsenic. Analysis of arsenic species is necessary in order to accurately assess arsenic exposure.
An analytical method was validated and used to determine and quantitate arsenic species in edible insects.
Arsenic species were extracted from edible insects by heating at 100°C in 0.3 mol/L nitric acid. The concentration of arsenic species was then determined by LC-inductively coupled plasma-mass spectrometry (LC-ICP-MS) using an octadecylsilane (ODS) column with a mobile phase containing an ion-pair reagent.
The LOD (0.007-0.012 mg/kg), LOQ (0.021-0.038 mg/kg), repeatability (1.2-3.2%), intermediate precision (2.8-4.5%), and trueness (recoveries 97-102% based on spiked samples) of the proposed method were satisfactory for inorganic arsenic, dimethylarsinic acid (DMA), and arsenobetaine (AB) in edible insects. Total arsenic was detected in all samples obtained in Japan (Asian forest scorpion, diving beetles, giant water bug, grasshoppers, June beetles, mole crickets, male rhino beetle, female rhino beetle, sago worms, and silkworm pupae) and consisted of mostly inorganic arsenic. Beetles in particular showed relatively high levels.
Arsenic content varies among edible insect species. Feed control is important, as arsenic concentrations in edible insects may be feed dependent.
Arsenic species in edible insects were analyzed by LC-ICP-MS using an ODS column with a mobile phase containing an ion-pair reagent. Inorganic arsenic was detected in most samples, with concentrations ranging from <0.04 to 29.3 mg/kg.
食用昆虫可能含有砷。为了准确评估砷暴露，需要分析砷的形态。
验证并应用一种分析方法来测定和定量食用昆虫中的砷形态。
将食用昆虫在 0.3mol/L 硝酸中于 100°C 加热提取砷形态。然后通过使用含有离子对试剂的流动相的反相高效液相色谱-电感耦合等离子体质谱（LC-ICP-MS）来测定砷形态的浓度。
该方法对食用昆虫中的无机砷、二甲基砷酸（DMA）和砷甜菜碱（AB）的检测限（0.007-0.012mg/kg）、定量限（0.021-0.038mg/kg）、重复性（1.2-3.2%）、中间精密度（2.8-4.5%）和准确度（基于加标样品的回收率为 97-102%）均令人满意。在日本获得的所有样品（亚洲雨林蝎、潜水甲虫、大水黾、蝗虫、六月甲虫、蝼蛄、雄性独角仙、雌性独角仙、西米虫和蚕蛹）中均检测到总砷，且主要由无机砷组成。特别是甲虫中的砷含量相对较高。
食用昆虫的砷含量因物种而异。饲料控制很重要，因为食用昆虫中的砷浓度可能取决于饲料。
使用含有离子对试剂的流动相的反相高效液相色谱-电感耦合等离子体质谱（LC-ICP-MS）分析食用昆虫中的砷形态。大多数样品中均检测到无机砷，浓度范围为<0.04-29.3mg/kg。
