Department of Endocrinology, First Affiliated Hospital of Gannan Medical University, Ganzhou City, Jiangxi Province, China.
J Physiol Pharmacol. 2023 Apr;74(2). doi: 10.26402/jpp.2023.2.03. Epub 2023 Jul 10.
Insulin resistance (IR) is predominantly causal for type 2 diabetes mellitus (T2DM). To solve this problem, this study particularly determined the role of quercetin (Que) in controlling IR in T2DM mice. The T2DM mouse model was established, and given 20 mg/kg/d Que by gavage for 6 weeks, and the lentiviral vector that interfered with microRNA-92b-3p (miR-92b-3p) or early growth response 1 (EGR1) expression was injected into the tail vein of T2DM mice. Blood glucose homeostasis and histopathological changes in the pancreas were observed after the corresponding treatment. miR-92b-3p and EGR1 expressions were assessed in T2DM mice, as well as their interlink. In results we found that Que could improve IR and pancreatic histopathological changes in T2DM mice. Low miR-92b-3p and high EGR1 were expressed in T2DM mice, while Que could upregulate miR-92b-3p to target EGR1. Enhancing miR-92b-3p or reducing EGR1 could further improve IR and pancreatic histopathological changes in T2DM mice after Que administration. Nevertheless, silencing miR-92b-3p or overexpressing EGR1 contributed to the opposite results. We concluded that Que exerted anti-diabetic effects in T2DM mice by regulating the miR-92b-3p/EGR1 axis.
胰岛素抵抗（IR）是 2 型糖尿病（T2DM）的主要病因。为了解决这个问题，本研究特别确定了槲皮素（Que）在控制 T2DM 小鼠 IR 中的作用。建立了 T2DM 小鼠模型，并通过灌胃给予 20mg/kg/d Que 6 周，将干扰 microRNA-92b-3p（miR-92b-3p）或早期生长反应 1（EGR1）表达的慢病毒载体注入 T2DM 小鼠的尾静脉。在相应的治疗后观察血糖稳态和胰腺的组织病理学变化。评估了 T2DM 小鼠中 miR-92b-3p 和 EGR1 的表达及其相互关系。结果发现，Que 可改善 T2DM 小鼠的 IR 和胰腺组织病理学变化。T2DM 小鼠中低表达 miR-92b-3p 和高表达 EGR1，而 Que 可以上调 miR-92b-3p 以靶向 EGR1。增强 miR-92b-3p 或降低 EGR1 可以进一步改善 Que 给药后 T2DM 小鼠的 IR 和胰腺组织病理学变化。然而，沉默 miR-92b-3p 或过表达 EGR1 则会产生相反的结果。我们得出结论，Que 通过调节 miR-92b-3p/EGR1 轴在 T2DM 小鼠中发挥抗糖尿病作用。
