College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi 830017, China; Xinjiang Bingtuan Xingxin Vocational and Technical College, Urumqi 830074, China.
College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi 830017, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 5;302:123094. doi: 10.1016/j.saa.2023.123094. Epub 2023 Jun 30.
We report a low-cost and highly sensitive label-free SERS biosensor for pathogen detection. Herein, this study prepared 4-formylphenylboric acid (FPBA) functionalized magnetic nanoparticles to adsorb pathogenic bacteria through boric acid affinity principle, and used aptamer modified Au@AgNPs as SERS substrate to specifically combine with pathogenic bacteria to form a sandwich structure. The pathogenic bacteria were detected by portable Raman spectrometer for SERS detection, and the fingerprint signals of pathogenic bacteria were analyzed by principal component analysis (PCA) to achieve the purpose of classification and identification of pathogenic bacteria. Under the optimized conditions, the SERS detection of Staphylococcus aureus (S. aureus) was 10 ∼ 10 CFU/mL (R = 0.9925), and the limit of detection (LOD) was 34 CFU/mL. The linear range of Escherichia coli (E. coli) showed a good linear relationship in the range of 10 ∼ 10 CFU/mL (R = 0.9993), and the LOD was 18 CFU/mL. The whole detection process was used the portable Raman spectrometer, which makes it suitable for the application of point-of-care testing (POCT).
我们报道了一种低成本、高灵敏度的无标记表面增强拉曼散射（SERS）生物传感器，用于病原体检测。在这项研究中，我们制备了 4-甲酰基苯硼酸（FPBA）功能化的磁性纳米粒子，通过硼酸亲和原理吸附致病菌，并使用适配体修饰的 Au@AgNPs 作为 SERS 基底，特异性地与致病菌结合形成三明治结构。使用便携式拉曼光谱仪进行 SERS 检测，通过主成分分析（PCA）对致病菌的指纹信号进行分析，从而达到对致病菌进行分类和鉴定的目的。在优化条件下，对金黄色葡萄球菌（S. aureus）的 SERS 检测下限（LOD）为 34 CFU/mL，检测范围为 10 ～ 10 CFU/mL（R = 0.9925）。对大肠杆菌（E. coli）的检测线性范围为 10 ～ 10 CFU/mL（R = 0.9993），LOD 为 18 CFU/mL。整个检测过程使用便携式拉曼光谱仪，使其适用于即时检测（POCT）的应用。
