Key Laboratory of Hainan Trauma and Disaster Rescue, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, 571199, China; Engineering Research Center for Hainan Bio-Smart Materials and Bio-Medical Devices, Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Functional Materials and Molecular Imaging, College of Emergency and Trauma, Hainan Medical University, Haikou, 571199, China; Jiangyin Center for Disease Control and Prevention, No. 158 Changjiang Road, Jiangyin, 214431, China.
School of Pharmacy, Hainan Medical University, Haikou, 571199, China.
Talanta. 2024 Dec 1;280:126691. doi: 10.1016/j.talanta.2024.126691. Epub 2024 Aug 8.
Staphylococcus aureus (S. aureus) is the most common pathogen in human purulent infections, which can cause local purulent infections, as well as pneumonia, pseudomembranous enteritis, pericarditis, and even systemic infections. The conventional methods including bacteria colony counting, polymerase chain reaction and enzyme-linked immunosorbent assay can't fully meet the requirement of highly sensitive detection of S. aureus due to their own disadvantages. Therefore, it's an urgent need to develop new platform to detect S. aureus in the early infection stage. In this study, a new surface-enhanced Raman scattering (SERS)-based nanoplatform based on dual-recognition of aptamer (Apt) and vancomycin (Van) was developed for the highly sensitive detection of S. aureus. The SERS nanoplatform consisted of two functional parts: aptamer-conjugated FeO magnetic nanoparticles (FeO-Apt MNPs) for bacteria enrichment and vancomycin modified-Au nanoparticles (Van-Au NPs) as the SERS probes for S. aureus quantitative detection. Upon the target bacteria enrichment, the SERS signals of the supernatant after magnetic separation could be obtained and analyzed under different concentrations of S. aureus. The limit of detection of the proposed assay was found to be 3.27 CFU/mL. We believe that the proposed SERS-based nanoplatform has great potential as a powerful tool in the early detection of specific bacteria.
金黄色葡萄球菌（S. aureus）是人类化脓性感染中最常见的病原体，可引起局部化脓性感染，以及肺炎、伪膜性肠炎、心包炎，甚至全身感染。由于自身的缺点，传统的方法包括细菌菌落计数、聚合酶链反应和酶联免疫吸附试验，无法完全满足对金黄色葡萄球菌进行高灵敏度检测的要求。因此，迫切需要开发新的平台来在早期感染阶段检测金黄色葡萄球菌。在这项研究中，开发了一种基于适配体（Apt）和万古霉素（Van）双重识别的新型表面增强拉曼散射（SERS）纳米平台，用于高灵敏度检测金黄色葡萄球菌。SERS 纳米平台由两个功能部分组成：用于细菌富集的适配体偶联的 FeO 磁性纳米颗粒（FeO-Apt MNPs）和作为 S. aureus 定量检测的万古霉素修饰的金纳米颗粒（Van-Au NPs）。在目标细菌富集后，可在不同浓度的金黄色葡萄球菌下获得磁分离后上清液的 SERS 信号，并进行分析。该方法的检测限被发现为 3.27 CFU/mL。我们相信，所提出的基于 SERS 的纳米平台具有作为特定细菌早期检测的强大工具的巨大潜力。
