The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Cisen Pharmaceutical Co., Ltd., Jining, 272073, China.
Talanta. 2022 Dec 1;250:123648. doi: 10.1016/j.talanta.2022.123648. Epub 2022 Jun 3.
An ultrasensitive and dual-recognition surface-enhanced Raman scattering (SERS) biosensor for Staphylococcus aureus (S. aureus) was constructed, which was based on teicoplanin (Tcp) functionalized gold-coated magnet nanoparticles (FeO@Au-Tcp NPs) as capture probe and S. aureus aptamer (Apt) functionalized silver coated gold nanoparticles (Au@Ag-DTNB-Apt NPs) as signal probe. Both Au NPs and Au@Ag NPs were prepared by a green synthesis method. Especially, the synthesis method of Au@Ag NPs reduced by chitosan (CS) was first reported in this work. Due to the great SERS enhancement based on the hot spot effect between Au NPs and Au@Ag NPs, and the dual-recognition ability based on Tcp and Apt, the SERS biosensor was ultrasensitive and specific. A detection limit of 1.09 CFU mL with a broad dynamic linear (7.6 × 10-7.6 × 10 CFU mL) was achieved within 50 min without interference by other bacteria. Moreover, the SERS biosensor could be applied for detection of S. aureus in milk and orange juice samples. This study provides a green, rapid and ultrasensitive method to detect S. aureus, and also explores the high utilization value of CS and Tcp, which has a broad application prospect in detection of pathogenic bacteria.
构建了一种基于头孢地尼（Tcp）功能化金包覆磁纳米粒子（FeO@Au-Tcp NPs）作为捕获探针和金黄色葡萄球菌适配体（Apt）功能化银包覆金纳米粒子（Au@Ag-DTNB-Apt NPs）作为信号探针的超灵敏双识别表面增强拉曼散射（SERS）生物传感器。两种 Au NPs 和 Au@Ag NPs 均采用绿色合成方法制备。特别是，本文首次报道了壳聚糖（CS）还原法制备 Au@Ag NPs。由于基于 Au NPs 和 Au@Ag NPs 之间的热点效应的巨大 SERS 增强，以及基于 Tcp 和 Apt 的双识别能力，该 SERS 生物传感器具有超灵敏和特异性。在 50 分钟内，无需其他细菌干扰，即可实现检测限为 1.09 CFU mL，动态线性范围为 7.6×10-7.6×10 CFU mL。此外，该 SERS 生物传感器可用于检测牛奶和橙汁样品中的金黄色葡萄球菌。本研究提供了一种绿色、快速和超灵敏的检测金黄色葡萄球菌的方法，同时也探索了 CS 和 Tcp 的高利用价值，在致病菌检测方面具有广阔的应用前景。
