Hermans Jos, Alipour Najmi Ali, Permentier Hjalmar, Bischoff Rainer
Department of Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, the Netherlands.
Department of Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, the Netherlands.
Talanta. 2024 Jan 1;266(Pt 1):124928. doi: 10.1016/j.talanta.2023.124928. Epub 2023 Jul 11.
Flow cell reactors used for catalyst development and applications are upcoming due to their small environmental and economic footprint. Online microflow reactor coupling with mass spectrometry (MS) opens new possibilities for monitoring catalyst performance and identifying reaction products in real time. This is demonstrated for the metabolic relevant dealkylation of lidocaine on catalytic gold micro-particles using regular liquid chromatography modules. Yields of up to 90% norlidocaine were realized under mild continuous flow conditions for up to 10 h (pH 7, 30 °C and 20 μL/min). Dissolved oxygen was shown to be a rate-limiting factor, since an inline oxygen generator allowed to increase the reactor capacity by one order of magnitude. Monitoring product time-response curve slopes after starting and ending a substrate feed, provided insights into the adsorption/desorption and conversion kinetics at the catalyst surface indicating the presence of strong adsorption sites that do not contribute substantially to substrate conversion.
由于其对环境和经济的影响较小，用于催化剂开发和应用的流动池反应器即将问世。在线微流反应器与质谱联用（MS）为实时监测催化剂性能和识别反应产物开辟了新的可能性。使用常规液相色谱模块，对利多卡因在催化金微粒上与代谢相关的脱烷基反应进行了验证。在温和的连续流动条件下（pH 7、30°C和20μL/min），长达10小时可实现高达90%的去甲利多卡因产率。溶解氧被证明是一个限速因素，因为在线氧气发生器可使反应器容量提高一个数量级。监测底物进料开始和结束后产物的时间响应曲线斜率，有助于深入了解催化剂表面的吸附/解吸和转化动力学，表明存在对底物转化贡献不大的强吸附位点。
