Abbas Muhammad, Alossaimi Manal A, Altamimi Abdulmalik S A, Alajaji Mai, Watson David G, Shah Sayyed I, Shah Yasar, Anwar Mohammad S
Department of Pharmacy, Abdul Wali Khan University Mardan, Mardan, Pakistan.
Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam Bin Abdul Aziz University, Al-Kharj, Saudi Arabia.
Front Pharmacol. 2023 Jun 26;14:1145962. doi: 10.3389/fphar.2023.1145962. eCollection 2023.
This study was performed to determine the levels of α1-acid glycoprotein (AGP) in old-age patients undergoing total hip arthroplasty. AGP is considered an acute phase protein produced during the acute phase reaction in the body to various stimuli; their proper monitoring is thus important. In order to study how AGP concentrations in old age patients change in response to surgical stress (total hip arthroplasty), a high-performance liquid chromatography assay was performed to measure AGP levels. AGP was isolated from the plasma by adding perchloric acid and was analyzed using PLRP-S 4000°A column. The mobile phase consisted of 1 mL TFA/L of water (Solvent A pH 2) and 1 mL TFA/L of acetonitrile (Solvent B). The gradient used was as follows: 0 min 18% B and 82% A, 15 min 60% B and 40% A, and 17 min 60% B and 40% A followed by column re-equilibration for 7 min before the next injection. AGP peak was obtained between 8.8 and 8.9 min. The method was fully optimised according to established guidelines. The data obtained were analyzed on ChromQuest software. AGP concentrations were determined in all samples, including baseline and samples taken at different timed intervals. The peak for AGP was obtained between 8.8 and 8.9 min for both standard AGP and patient plasma. The graphs indicate that AGP concentration in almost all patient samples increased considerably, especially after 4 h and 24 h-for example, initial concentration in patient 1 was 10.36 mg/100 mL but, after 24 h, increased to 23.50 mg/100 mL. There was thus almost a 13 mg/100 mL increase in 24 h, which is confirmed by AGP concentration increasing after various conditions, including surgery. The increased plasma protein binding was comparatively associated with the unchanged free fraction of the drug. This surgically induced increase in AGP concentration resulted in increased plasma protein binding of the drug (ropivacaine), which in turn kept the free portion of ropivacaine stable during the postoperative period.
本研究旨在测定接受全髋关节置换术的老年患者体内α1-酸性糖蛋白（AGP）的水平。AGP被认为是机体在急性期反应中针对各种刺激产生的一种急性期蛋白；因此，对其进行适当监测很重要。为了研究老年患者体内AGP浓度如何因手术应激（全髋关节置换术）而变化，采用高效液相色谱法测定AGP水平。通过加入高氯酸从血浆中分离出AGP，并使用PLRP-S 4000°A柱进行分析。流动相由1mL三氟乙酸/升水（溶剂A，pH 2）和1mL三氟乙酸/升乙腈（溶剂B）组成。所用梯度如下：0分钟时为18%B和82%A，15分钟时为60%B和40%A，17分钟时为60%B和40%A，随后在下次进样前进行7分钟的柱重新平衡。AGP峰在8.8至8.9分钟之间出现。该方法已根据既定指南进行了充分优化。所获得的数据在ChromQuest软件上进行分析。测定了所有样本中的AGP浓度，包括基线样本和在不同时间间隔采集的样本。标准AGP和患者血浆的AGP峰均在8.8至8.9分钟之间出现。图表显示，几乎所有患者样本中的AGP浓度都显著增加，尤其是在4小时和24小时后——例如，患者1的初始浓度为10.36mg/100mL，但在24小时后增加到23.50mg/100mL。因此，24小时内几乎增加了13mg/100mL，这在包括手术在内的各种情况下AGP浓度增加得到了证实。血浆蛋白结合增加与药物游离部分未改变相对相关。这种手术诱导的AGP浓度增加导致药物（罗哌卡因）的血浆蛋白结合增加，这反过来又使罗哌卡因的游离部分在术后期间保持稳定。
