Saghir Sultan A M, Alnaimat Sulaiman M, Dmour Saif M, Al-Tarawni Ayat H, Abdelnour Sameh A, Ahmeda Ahmad F, Arisha Ahmed H, Hawwal Mohammed F, Alanzi Abdullah R, Mothana Ramzi A, Lindequist Ulrike
Department of Medical Analysis. Princess Aisha Bint Al-Hussein College of Nursing and Medical Sciences, Al-Hussein Bin Talal University, Ma'an 71111, Jordan.
Department of Biology Department, Al-Hussein Bin Talal University, Ma'an, Jordan.
Saudi Pharm J. 2023 Aug;31(8):101691. doi: 10.1016/j.jsps.2023.06.024. Epub 2023 Jun 28.
This work explored the activities of bergamot oil nano-emulsion (NBG) in modulating blood biochemical parameters, redox status, immunity indices, inflammation markers, semen quality, testicular changes and the expression of HSPs genes in stressed rabbit bucks. Twenty-four mature rabbit bucks (5 months) were randomly divided into three groups; control group (NBG0) received 1 ml of distilled water, while the other two groups received NBG orally at doses of 50 and 100 mg/kg (bw) twice a week. The present study's findings revealed that treated groups had lower values of total and direct bilirubin, triglyceride, lactate dehydrogenase, and creatinine compared with NBG0 group ( < 0.05). NBG100 group recorded the greatest of total protein, albumin, GPx, T3 and T4 values as well as the lowest values of uric acid, MDA, and indirect bilirubin. Both treated groups showed significantly reduced 8-OhDG, Amyloid A, TLR 4, while significantly increased nitric oxide, IgA, IgM, TAC, and SOD levels. Semen characteristics such as volume, sperm count, sperm motility, normal sperm, and vitality were significantly higher in the NBG100 group compared to the NBG50 and NBG0 groups, whereas sperm abnormalities and dead sperm were significantly reduced. HSP70, HSP72, and HSPA9 gene overexpression showed that testicular integrity was maintained after buck received oral doses of 50 or 100 mg/kg of NBG. Existing findings indicate that oral administration of NBG improves heat tolerance in rabbit bucks primarily as e result of its antioxidant and anti-inflammatory effects.
本研究探讨了佛手柑油纳米乳剂（NBG）对受应激雄兔血液生化参数、氧化还原状态、免疫指标、炎症标志物、精液质量、睾丸变化及热休克蛋白（HSPs）基因表达的影响。将24只5月龄成熟雄兔随机分为三组；对照组（NBG0）给予1ml蒸馏水，另外两组每周两次口服50和100mg/kg（体重）剂量的NBG。本研究结果显示，与NBG0组相比，治疗组的总胆红素、直接胆红素、甘油三酯、乳酸脱氢酶和肌酐值较低（P<0.05）。NBG100组的总蛋白、白蛋白、谷胱甘肽过氧化物酶（GPx）、三碘甲状腺原氨酸（T3）和甲状腺素（T4）值最高，而尿酸、丙二醛（MDA）和间接胆红素值最低。两个治疗组的8-羟基脱氧鸟苷（8-OhDG）、淀粉样蛋白A、Toll样受体4（TLR 4）均显著降低，而一氧化氮、免疫球蛋白A（IgA）、免疫球蛋白M（IgM）、总抗氧化能力（TAC）和超氧化物歧化酶（SOD）水平显著升高。与NBG50组和NBG0组相比，NBG100组的精液体积、精子数量、精子活力、正常精子率和精子活力等精液特征显著更高，而精子畸形率和死精率显著降低。热休克蛋白70（HSP70）、热休克蛋白72（HSP72）和热休克蛋白A9（HSPA9）基因的过表达表明，雄兔口服50或100mg/kg剂量的NBG后，睾丸完整性得以维持。现有研究结果表明，口服NBG主要通过其抗氧化和抗炎作用提高雄兔的耐热性。
