Lehmann J, Martin H L, Lashley E L, Marshall M W, Judd J T
J Am Diet Assoc. 1986 Sep;86(9):1208-16.
As part of a human diet study, vitamin E activity was estimated in foods used in seven daily menus. Each menu was designed to contain 35% fat calories with either 10 or 30 gm/day of linoleic acid (18:2) and 500 mg/day of cholesterol. To estimate vitamin E activity, each food used in the menus was analyzed for alpha and gamma tocopherol content by high-pressure liquid chromatography with fluorescence detection. This article reports the alpha and gamma tocopherol contents of those foods, tocopherol contributions from each food in one sample 2,400-kcal menu, and the mean daily vitamin E activity (milligram alpha tocopherol equivalents) of all seven menus at five caloric levels. Major sources of alpha tocopherol (greater than 10% of the RDA) common to both diets (10 and 30 gm linoleic acid) were olive oil and a few fruits and vegetables. Additional major sources in the 30-gm linoleic acid diets were polyunsaturated fatty acid (PUFA) vegetable oils and margarine. Contrary to a common assumption, increasing the level of PUFA in the menus did not necessarily result in higher milligram equivalents of alpha tocopherol because soybean oil, with a tocopherol composition that is predominantly gamma tocopherol, was the major source of linoleic acid in the diets. Thus, vitamin E activity was not necessarily increased when soybean oil was substituted for a less saturated fat such as olive oil, which has mostly alpha tocopherol.
作为一项人体饮食研究的一部分，对七份日常菜单中所使用食物的维生素E活性进行了评估。每份菜单的设计目标是脂肪热量占比35%，同时含有10克或30克/天的亚油酸（18:2）以及500毫克/天的胆固醇。为了评估维生素E活性，采用带荧光检测的高压液相色谱法对菜单中使用的每种食物的α-生育酚和γ-生育酚含量进行了分析。本文报告了这些食物的α-生育酚和γ-生育酚含量、一份2400千卡菜单中每种食物的生育酚贡献量，以及五个热量水平下所有七份菜单的每日平均维生素E活性（毫克α-生育酚当量）。两种饮食（10克和30克亚油酸）共有的α-生育酚主要来源（超过推荐膳食摄入量的10%）是橄榄油以及一些水果和蔬菜。30克亚油酸饮食中的其他主要来源是多不饱和脂肪酸（PUFA）植物油和人造黄油。与通常的假设相反，增加菜单中PUFA的含量并不一定会导致更高毫克当量的α-生育酚，因为生育酚成分主要是γ-生育酚的大豆油是这些饮食中亚油酸的主要来源。因此，当用大豆油替代饱和度较低的脂肪（如主要含α-生育酚的橄榄油）时，维生素E活性不一定会增加。
