Zhao Shuai, Jie Xiang, Ma Zhe, Wang Zheng, Zhang Jichuan, Li Yushi, Nie Qiuhai, Ma Yong
Center of Advanced Elastomer Materials, College of Material Science & Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
ACS Omega. 2023 Jun 27;8(27):24185-24197. doi: 10.1021/acsomega.2c07870. eCollection 2023 Jul 11.
rubber is considered the most ideal alternative source of natural rubber (NR). Extracting rubber from with high quality, low cost, and low pollution is the basis of commercial development. The roots were subjected to morphological observation and detailed compositional analysis. Scanning electron microscopy (SEM) images confirm that rubber filaments are physically entangled with plant tissues due to differences in molecular polarity. Compared with the traditional solvent rubber extraction process, a new rubber extraction process developed in this study, namely, the microbial extraction (″ME″) process, is less harmful to the environment and lower in cost. The ″ME″ process is divided into three steps: dilute acid pretreatment process, enzyme degradation process, and fermentation process. After each step is completed, the purity of rubber will gradually increase from 84.8% to 93.8 to 95.5%. The rubber finally obtained fully meets the requirements of the traditional rubber industry, especially the tire industry. Besides, the yield of biofuel ethanol, a by-product of cellulose fermentation, reaches 2.05 g/100 g of roots (dry weight), which can effectively reduce the production cost of rubber. In the rubber extraction process, microorganisms have little effect on the quality of rubber. The results show that the molecular weight and chemical structure of rubber is very close to , so the ″ME″ process can be used as a new method for large-scale extraction of rubber.
杜仲胶被认为是天然橡胶（NR）最理想的替代来源。以高质量、低成本和低污染的方式从杜仲中提取橡胶是其商业开发的基础。对杜仲根进行了形态观察和详细的成分分析。扫描电子显微镜（SEM）图像证实，由于分子极性的差异，橡胶丝与植物组织物理缠绕在一起。与传统的溶剂萃取橡胶工艺相比，本研究开发的一种新的橡胶萃取工艺，即微生物萃取（“ME”）工艺，对环境危害更小且成本更低。“ME”工艺分为三个步骤：稀酸预处理工艺、酶降解工艺和发酵工艺。每一步完成后，杜仲胶的纯度将从84.8%逐渐提高到93.8%再到95.5%。最终得到的杜仲胶完全符合传统橡胶工业尤其是轮胎工业的要求。此外，纤维素发酵的副产品生物燃料乙醇的产量达到2.05 g/100 g杜仲根（干重），这可以有效降低杜仲胶的生产成本。在橡胶萃取过程中，微生物对杜仲胶的质量影响很小。结果表明，杜仲胶的分子量和化学结构与天然橡胶非常接近，因此“ME”工艺可作为大规模提取杜仲胶的一种新方法。
